{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:57:56Z","timestamp":1777633076562,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S)\u2010releasing agents are viewed as potential antihypertensive drugs. Recently, natural isothiocyanates emerged as original H<jats:sub>2<\/jats:sub>S\u2010donor agents. Among them, erucin, present in some edible cruciferous plants, shows suitable H<jats:sub>2<\/jats:sub>S\u2010releasing properties and features of \u201cdruggability.\u201d The aim of this work was to investigate the erucin\u2010mediated release of H<jats:sub>2<\/jats:sub>S inside vascular cells, its vasorelaxing effects, and activity on BP of normo and hypertensive animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Intracellular H<jats:sub>2<\/jats:sub>S\u2010release and the hyperpolarizing effect of erucin were tested using fluorescent dye, in human aortic smooth muscle cells (HASMCs). Its direct vasorelaxing effect and ability to inhibit noradrenaline\u2010induced vasoconstriction were evaluated on endothelium\u2010intact or \u2010denuded rat aortic rings. Its vasodilator properties were tested in coronary arteries using Langendorff\u2010perfused rat hearts. Finally, erucin's antihypertensive activity was evaluated in vivo in normotensive and spontaneously hypertensive rats (SHRs) by recording systolic BP using the tail\u2010cuff method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Erucin induced the release of H<jats:sub>2<\/jats:sub>S inside HASMCs. Moreover, erucin hyperpolarized the membrane of HASMCs membrane in a concentration\u2010dependent manner. It induced vasodilatation of rat aortic rings, in endothelium\u2010denuded vessels. This effect was further improved by the presence of endothelial NO. When pre\u2010incubated with rat aortic rings, erucin induced concentration\u2010dependent inhibition of noradrenaline\u2010induced vasoconstriction. Erucin did not affect basal coronary flow but restored the flow to normal in pre\u2010contracted coronary vessels. Finally, in vivo, erucin decreased systolic BP in SHRs by about 25%, and restored the BP to values observed in normotensive rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Erucin is an H<jats:sub>2<\/jats:sub>S donor endowed with vasorelaxing and antihypertensive effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Linked Articles<\/jats:title><jats:p>This article is part of a themed section on Hydrogen Sulfide in Biology &amp; Medicine. To view the other articles in this section visit <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v177.4\/issuetoc\">http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v177.4\/issuetoc<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14645","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T14:43:29Z","timestamp":1551537809000},"page":"824-835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Erucin exhibits vasorelaxing effects and antihypertensive activity by H<sub>2<\/sub>S\u2010releasing properties"],"prefix":"10.1111","volume":"177","author":[{"given":"Alma","family":"Martelli","sequence":"first","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Centre \u201cNutraceuticals and Food for Health (NUTRAFOOD)\u201d University 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