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This question was addressed in the present study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Wild\u2010type mice and mice with cardiac transgenic expression of \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptors, mammalian sterile\u201020 like kinase 1 (Mst1) or dominant\u2010negative Mst1, and non\u2010specific galectin\u20103 knockout mice were used. Effects of the \u03b2\u2010adrenoceptor agonist isoprenaline or \u03b2\u2010adrenoceptor antagonists were studied. Rat cardiomyoblasts (H9c2) were used for mechanistic exploration. Biochemical assays were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Isoprenaline treatment up\u2010regulated expression of galectin\u20103 and BIM, and this was inhibited by non\u2010selective or selective \u03b2\u2010adrenoceptor antagonists (by 60\u201370%). Cardiac expression of galectin\u20103 and BIM was increased in \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptor transgenic mice. Isoprenaline\u2010induced up\u2010regulation of galectin\u20103 and BIM was attenuated by Mst1 inactivation, but isoprenaline\u2010induced galectin\u20103 expression was exaggerated by transgenic Mst1 activation. Pharmacological or genetic activation of \u03b2\u2010adrenoceptors induced Mst1 expression and yes\u2010associated protein (YAP) phosphorylation. YAP hyper\u2010phosphorylation was also evident in Mst1 transgenic hearts with up\u2010regulated expression of galectin\u20103 (40\u2010fold) and BIM as well as up\u2010regulation of many YAP\u2010target genes by RNA sequencing. In H9c2 cells, isoprenaline induced YAP phosphorylation and expression of galectin\u20103 and BIM, effects simulated by forskolin but abolished by PKA inhibitors, and YAP knockdown induced expression of galectin\u20103 and BIM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Stimulation of cardiac \u03b2\u2010adrenoceptors activated the Mst1\/Hippo pathway leading to YAP hyper\u2010phosphorylation with enhanced expression of galectin\u20103 and BIM. This signalling pathway would have therapeutic potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Linked Articles<\/jats:title><jats:p>This article is part of a themed section on Adrenoceptors\u2014New Roles for Old Players. To view the other articles in this section visit <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v176.14\/issuetoc\">http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v176.14\/issuetoc<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14674","type":"journal-article","created":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T09:08:31Z","timestamp":1554109711000},"page":"2465-2481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Stimulation of \u03b2\u2010adrenoceptors up\u2010regulates cardiac expression of galectin\u20103 and <scp>BIM<\/scp> through the <scp>H<\/scp>ippo signalling pathway"],"prefix":"10.1111","volume":"176","author":[{"given":"Wei\u2010Bo","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Cardiology, Southwest Hospital Third Military Medical University (Army Medical University)  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