{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:25:21Z","timestamp":1790375121489,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T00:00:00Z","timestamp":1558051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773556"],"award-info":[{"award-number":["81773556"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010096","name":"Southern Medical University","doi-asserted-by":"publisher","award":["B1040903"],"award-info":[{"award-number":["B1040903"]}],"id":[{"id":"10.13039\/501100010096","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Propionibacterium acnes<\/jats:italic><\/jats:styled-content> is a Gram\u2010positive bacterium associated with the skin disorder acne. In this study, as fatty acids are considered to be important in the life habitat of <jats:italic>P.\u00a0acnes<\/jats:italic>, we tested our lipopeptide library in an attempt to create potent <jats:italic>P.\u00a0acnes<\/jats:italic>\u2010specific antimicrobial agents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>The antimicrobial activity of various lipopeptides was determined by measuring their minimal inhibitory concentration (MIC). Lipids from <jats:italic>P.\u00a0acnes<\/jats:italic> were used to explore their mode of action. RAW264.7 cells stimulated with LPS and <jats:italic>P.\u00a0acnes<\/jats:italic> respectively were used to measure their anti\u2010inflammatory activity. Mice ears injected with <jats:italic>P.\u00a0acnes<\/jats:italic> were used to assess the antimicrobial and anti\u2010inflammatory effects of the peptides tested in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The most potent candidate, C16\u2010KWKW, was observed to be more active against <jats:italic>P.\u00a0acnes<\/jats:italic> than against other non\u2010targeted bacterial strains, such as <jats:italic>Streptococcus mutans<\/jats:italic>, <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic>,<\/jats:styled-content> and <jats:italic><jats:styled-content style=\"fixed-case\">Escherichia coli<\/jats:styled-content><\/jats:italic>. The mode of action of C16\u2010KWKW was observed to be through interference with the integrity of the bacterial membrane, thereby impairing membrane permeability and causing leakage of inner contents of bacterial cells. Furthermore, C16\u2010KWKW inhibited the expression of pro\u2010inflammatory cytokines, such as IL\u20101\u03b2, TNF\u2010\u03b1, and inducible NOS stimulated by both LPS and <jats:italic>P.\u00a0acnes<\/jats:italic>, thus showing potential anti\u2010inflammatory activity, which was further verified in the in vivo animal studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>C16\u2010KWKW is a lipopeptide displaying both anti\u2010<jats:italic>P.\u00a0acnes<\/jats:italic> and anti\u2010inflammatory effects in vitro and in vivo and shows potential as a treatment for acne vulgaris induced by <jats:italic>P.\u00a0acnes<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14680","type":"journal-article","created":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T12:53:21Z","timestamp":1553950401000},"page":"2321-2335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Short lipopeptides specifically inhibit the growth of <i>Propionibacterium acnes<\/i> with a dual antibacterial and anti\u2010inflammatory action"],"prefix":"10.1111","volume":"176","author":[{"given":"Guang","family":"Yang","sequence":"first","affiliation":[{"name":"Group of Peptides and Natural Products Research, School of Pharmaceutical Sciences Southern Medical University  Guangzhou P.R. 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