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Benzimidazole derivatives exhibit broad\u2010spectrum pharmacological properties including anti\u2010microbial, anti\u2010diabetic and anti\u2010tumour activity. However, whether benzimidazole derivatives are effective in suppressing angiogenesis and its underlying mechanisms remain incompletely understood. In this study, we aim to characterize the anti\u2010angiogenic mechanisms of a novel 2\u2010aminobenzimidazole\u2010based compound, Jzu 17, in an effort to develop novel angiogenesis inhibitor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Effects of Jzu 17 on endothelial cell proliferation, migration, invasion, and activation of signalling molecules induced by VEGF\u2010A, were analysed by immunoblotting, MTT, BrdU, migration, and invasion assays. We performed tube formation assay, aorta ring sprouting assay, matrigel plug assay, and a mouse model of metastasis to evaluate ex vivo and in vivo anti\u2010angiogenic effects of Jzu 17.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Jzu 17 inhibited VEGF\u2010A\u2010induced cell proliferation, migration, invasion, and endothelial tube formation of HUVECs. Jzu 17 suppressed VEGF\u2010A\u2010induced microvessel sprouting ex vivo and attenuated VEGF\u2010A\u2010 or tumour cell\u2010induced neovascularization in vivo. Jzu 17 also reduced B16F10 melanoma lung metastasis. In addition, Jzu 17 inhibited the phosphorylation of VEGFR\u20102 and its downstream signalling molecules in VEGF\u2010A\u2010stimulated HUVECs. Results from computer modelling further showed that Jzu 17 binds to VEGFR\u20102 with high affinity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Jzu 17 may inhibit endothelial remodelling and suppress angiogenesis through targeting VEGF\u2010A\u2010VEGFR\u20102 signalling. These results also suggest Jzu 17 as a potential lead compound and warrant the clinical development of similar agents in the treatment of cancer and angiogenesis\u2010related diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14813","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T06:13:02Z","timestamp":1564639982000},"page":"4034-4049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A novel 2\u2010aminobenzimidazole\u2010based compound Jzu 17 exhibits anti\u2010angiogenesis effects by targeting VEGFR\u20102 signalling"],"prefix":"10.1111","volume":"176","author":[{"given":"Jin\u2010Cherng","family":"Lien","sequence":"first","affiliation":[{"name":"School of Pharmacy China Medical University  Taichung Taiwan"},{"name":"Department of Medical Research Hospital of China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0656-976X","authenticated-orcid":false,"given":"Chi\u2010Li","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Internal Medicine Taipei Medical University Hospital  Taipei Taiwan"},{"name":"Division of Thoracic Medicine, Department of Internal Medicine, School of Medicine and School of Respiratory Therapy, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tur\u2010Fu","family":"Huang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Pharmacology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsung\u2010Chia","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Pharmacy China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuan\u2010Chung","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ging\u2010Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmacy China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9688-8494","authenticated-orcid":false,"given":"Ming\u2010Jen","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Cell Physiology and Molecular Image Research Center, Wan Fang Hospital Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiu\u2010Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Department of Medical Research Taipei Medical University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13877"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13882"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-015-0310-8"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PPO.0000000000000138"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01582"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.540040211"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-390922"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2008.06.012"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2016-0062"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2015.1071350"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000088478"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.25"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14153"},{"key":"e_1_2_11_15_1","first-page":"399","article-title":"Benzimidazole derivatives as potential anticancer agents","volume":"13","author":"El Rashedy A. 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