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As inflammation is potentially involved in retinal degeneration, the pro\u2010inflammatory kallikrein\u2013kinin system is a possible alternative pharmacological target. Here, we investigated the effects of anti\u2010VEGF and anti\u2010B<jats:sub>1<\/jats:sub> receptor treatments on the inflammatory mechanisms in a rat model of choroidal neovascularization (CNV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Immediately after laser\u2010induced CNV, Long\u2013Evans rats were treated by eye\u2010drop application of a B<jats:sub>1<\/jats:sub> receptor antagonist (R\u2010954) or by intravitreal injection of B<jats:sub>1<\/jats:sub> receptor siRNA or anti\u2010VEGF antibodies. Effects of treatments on gene expression of inflammatory mediators, CNV lesion regression and integrity of the blood\u2010retinal barrier was measured 10 days later in the retina. B<jats:sub>1<\/jats:sub> receptor and VEGF\u2010R2 cellular localization was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The three treatments significantly inhibited the CNV\u2010induced retinal changes. Anti\u2010VEGF and R\u2010954 decreased CNV\u2010induced up\u2010regulation of B<jats:sub>1<\/jats:sub> and B<jats:sub>2<\/jats:sub> receptors, TNF\u2010\u03b1, and ICAM\u20101. Anti\u2010VEGF additionally reversed up\u2010regulation of VEGF\u2010A, VEGF\u2010R2, HIF\u20101\u03b1, CCL2 and VCAM\u20101, whereas R\u2010954 inhibited gene expression of IL\u20101\u03b2 and COX\u20102. Enhanced retinal vascular permeability was abolished by anti\u2010VEGF and reduced by R\u2010954 and B<jats:sub>1<\/jats:sub> receptor siRNA treatments. Leukocyte adhesion was impaired by anti\u2010VEGF and B<jats:sub>1<\/jats:sub> receptor inhibition. B<jats:sub>1<\/jats:sub> receptors were found on astrocytes and endothelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>B<jats:sub>1<\/jats:sub> receptor and VEGF pathways were both involved in retinal inflammation and damage in laser\u2010induced CNV. The non\u2010invasive, self\u2010administration of B<jats:sub>1<\/jats:sub> receptor antagonists on the surface of the cornea by eye drops might be an important asset for the treatment of AMD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14962","type":"journal-article","created":{"date-parts":[[2019,12,28]],"date-time":"2019-12-28T02:54:33Z","timestamp":1577501673000},"page":"1949-1966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The effects of anti\u2010VEGF and kinin B<sub>1<\/sub> receptor blockade on retinal inflammation in laser\u2010induced choroidal neovascularization"],"prefix":"10.1111","volume":"177","author":[{"given":"Soumaya","family":"Hachana","sequence":"first","affiliation":[{"name":"School of Optometry Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Quebec Canada"},{"name":"Department of Pharmacology and Physiology Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Quebec 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