{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:44:27Z","timestamp":1787496267169,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005026","name":"Stichting Tegen Kanker","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004497","name":"Onderzoeksraad, KU Leuven","doi-asserted-by":"publisher","award":["C1\u2010TRPLe"],"award-info":[{"award-number":["C1\u2010TRPLe"]}],"id":[{"id":"10.13039\/501100004497","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004727","name":"Vlaams Instituut voor Biotechnologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004727","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>The transient receptor potential (TRP) ion channel TRPM3 functions as a noxious heat sensor, plays a key role in acute pain sensation and inflammatory hyperalgesia in rodents. Despite its potential as a novel analgesic drug target, little is known about the expression, function and modulation in the humans.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>We studied TRPM3 in freshly isolated human dorsal root ganglion (hDRG) neurons and human stem cell\u2010derived sensory (hSCDS) neurons. Expression was analysed at the mRNA level using RT\u2010qPCR. Channel function was assessed using Fura\u20102\u2010based calcium imaging and whole\u2010cell patch\u2010clamp recordings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      TRPM3 was detected at the mRNA level in both hDRG and hSCDS neurons. The TRPM3 agonists pregnenolone sulphate (PS) and CIM0216 evoked robust intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      responses in 52% of hDRG and 58% of hSCDS neurons. Whole\u2010cell patch\u2010clamp recordings in hSCDS neurons revealed pregnenolone sulphate (PS)\u2010 and CIM0216\u2010evoked currents exhibiting the characteristic current\u2013voltage relation of TRPM3. PS\u2010induced calcium responses in hSCDS neurons were reversed in a dose\u2010dependent manner by the flavonoid isosakuranetin and by antiseizure drug primidone. Finally, the \u03bc\u2010opioid receptor agonist DAMGO and the GABA\n                      <jats:sub>B<\/jats:sub>\n                      receptor agonist baclofen inhibited PS\u2010evoked TRPM3 responses in a subset of hSCDS neurons.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>\n                      These results provide the first direct evidence of functional expression of the pain receptor TRPM3 in human sensory neurons, largely mirroring the channel's properties observed in mouse sensory neurons. hSCDS neurons represent a valuable and readily accessible\n                      <jats:italic>in vitro<\/jats:italic>\n                      model to study TRPM3 regulation and pharmacology in a relevant human cellular context.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.14994","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T05:16:39Z","timestamp":1580102199000},"page":"2683-2695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Functional expression and pharmacological modulation of TRPM3 in human sensory neurons"],"prefix":"10.1111","volume":"177","author":[{"given":"Laura","family":"Vangeel","sequence":"first","affiliation":[{"name":"Laboratory of Ion Channel Research VIB\u2010KU Leuven Center for Brain &amp; Disease Research  Leuven Belgium"},{"name":"Department of Cellular and Molecular Medicine KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Benoit","sequence":"additional","affiliation":[{"name":"Laboratory of Ion Channel Research VIB\u2010KU Leuven Center for Brain &amp; Disease Research  Leuven Belgium"},{"name":"Department of Cellular and Molecular Medicine KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Miron","sequence":"additional","affiliation":[{"name":"AnaBios  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Miller","sequence":"additional","affiliation":[{"name":"AnaBios  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrien","family":"De Clercq","sequence":"additional","affiliation":[{"name":"Laboratory of Ion Channel Research VIB\u2010KU Leuven Center for Brain &amp; Disease Research  Leuven Belgium"},{"name":"Department of Cellular and Molecular Medicine KU Leuven  Leuven Belgium"},{"name":"Laboratory of Endometrium, Endometriosis &amp; Reproductive Medicine, Department of Development and Regeneration, G\u2010PURE KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Chaltin","sequence":"additional","affiliation":[{"name":"Center for Drug Design and Discovery Bio\u2010Incubator 2  Heverlee Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Verfaillie","sequence":"additional","affiliation":[{"name":"Department of Development and Regeneration, Stem Cell Institute KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris","family":"Vriens","sequence":"additional","affiliation":[{"name":"Laboratory of Endometrium, Endometriosis &amp; Reproductive Medicine, Department of Development and Regeneration, G\u2010PURE KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5526-5821","authenticated-orcid":false,"given":"Thomas","family":"Voets","sequence":"additional","affiliation":[{"name":"Laboratory of Ion Channel Research VIB\u2010KU Leuven Center for Brain &amp; Disease Research  Leuven Belgium"},{"name":"Department of Cellular and Molecular Medicine KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14749"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14112"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.26147"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.023"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05910"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2018-0455"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3887"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/15360288.2015.1047553"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5464.306"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1529"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2249"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05676"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/35077544"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14153"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2014.06.017"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/35012076"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.26280"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.02.097"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41431-019-0462-x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-11-770"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1121"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419845112"},{"issue":"8","key":"e_1_2_11_26_1","first-page":"1","article-title":"The nociceptive TRPM3 channel as potential therapeutic target for chronic pain","volume":"3","author":"Held K.","year":"2017","journal-title":"Internal Medicine Review"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000478986"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-101011-155833"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808487106"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-3-39"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000846"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.03.042"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00892.x"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2014.123"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14044"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503092200"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.273581"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-017-1380-8"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2757"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.26138"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2017.11.047"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00240-4"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.113.086843"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature26137"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a035048"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2019.07.004"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1428"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3784"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.02.051"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1801"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3886"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.12703\/P7-56"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.87"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2014.86"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14702"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbph.14994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.14994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bph.14994","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.14994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T23:21:29Z","timestamp":1693956089000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.14994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,5]]},"references-count":54,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1111\/bph.14994"],"URL":"https:\/\/doi.org\/10.1111\/bph.14994","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,5]]},"assertion":[{"value":"2019-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}