{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:25:30Z","timestamp":1771953930778,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T00:00:00Z","timestamp":1600560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671639"],"award-info":[{"award-number":["81671639"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Epidemiological and experimental studies suggest that microbial exposure in early childhood is linked with reduced risk to suffer asthma. Thus microbial components with immunoregulatory capabilities might serve as a preventive strategy for allergic asthma. Recently, it was identified that <jats:styled-content style=\"fixed-case\"><jats:italic>Streptococcus pneumoniae<\/jats:italic><\/jats:styled-content> aminopeptidase N (PepN) could suppress T cell effector function. We sought to investigate the effect of PepN on murine allergic asthma and elucidate the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>The effects of intranasal administration of PepN during or before sensitization were examined in ovalbumin (OVA)\u2010induced murine allergic asthma. The roles of CD11b<jats:sup>+<\/jats:sup> dendritic cells in PepN treated OVA\u2010induced allergic asthma were evaluated by flow cytometry, cytokines detection and adoptive transfer. Moreover, the numbers of lung type 2 innate lymphoid cells (ILC2s) were also detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Administration of PepN during or before sensitization attenuated type\u20102 airway inflammation (eosinophilia, mucus hypersecretion, Th2 cytokines production and IgE production) in allergic asthma mice. PepN reduced lung accumulation of CD11b<jats:sup>+<\/jats:sup> dendritic cells, which was accompanied by diminished dendritic cell\u2010attracting chemokine CCL20 production as well as CCL17 and CCL22, which are Th2\u2010cell chemokines predominantly produced by CD11b<jats:sup>+<\/jats:sup> dendritic cells. Adoptive transfer of BM\u2010derived CD11b<jats:sup>+<\/jats:sup> dendritic cells abolished the inhibitory effect of PepN on OVA\u2010induced type\u20102 airway inflammation. The numbers of lung ILC2s were decreased in asthmatic mice receiving PepN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>PepN alleviated type\u20102 inflammation in OVA\u2010induced allergic asthma mice, which was mediated by regulation of lung CD11b<jats:sup>+<\/jats:sup> dendritic cells. Our study provides a novel strategy for the prevention of allergic asthma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.15216","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T18:33:50Z","timestamp":1596047630000},"page":"5063-5077","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<scp><i>Streptococcus pneumoniae<\/i><\/scp> aminopeptidase N regulates dendritic cells that attenuates type\u20102 airway inflammation in murine allergic asthma"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6885-936X","authenticated-orcid":false,"given":"Guangying","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si","family":"Sun","sequence":"additional","affiliation":[{"name":"The First People's Hospital of Chongqing Liang Jiang New Area  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhong","family":"Qi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibing","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0762-4479","authenticated-orcid":false,"given":"Wenchun","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14752"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12861"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/147323000503300503"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2015.05.017"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.3389\/fimmu.2017.01610","article-title":"A novel function for the Streptococcus pneumoniae aminopeptidase N: Inhibition of T cell effector function through regulation of TCR signaling","volume":"8","author":"Blevins L. 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