{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:21:51Z","timestamp":1777429311028,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Psoriasis is a chronic immune\u2010mediated inflammatory skin disease that easily recurs and is difficult to cure. DGT is a novel synthetic heterocyclic diterpenoid, whose structure has not been previously reported. We have investigated the action of DGT against psoriasis, specifically the hyperproliferation of epidermal keratinocytes, angiogenesis and pathogenic inflammatory responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>We investigated its pharmacokinetics in skin after topical administration. We characterized its pharmacological actions <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> using cell proliferation assay, cell apoptosis assay, diethylstilbestrol\u2010induced mouse vaginal epithelial cell mitosis model, tube formation assay, cell migration assay, chick embryonic chorioallantoic membrane (CAM) assay, histological, flow cytometric analysis and imiquimod (IMQ)\u2010induced psoriasis\u2010like model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>DGT was found to be mainly distributed in the epidermis and dermis, which indicated that DGT was suitable as a topical treatment. DGT inhibited cell proliferation and induced apoptotic cell death of keratinocytes <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. Moreover, DGT inhibited endothelial cell proliferation, tube formation and migration of <jats:italic>in vitro<\/jats:italic> angiogenesis, as well as <jats:italic>in vivo<\/jats:italic> CAM angiogenesis. In an IMQ\u2010induced psoriasis\u2010like skin inflammation murine model, topical application of DGT ameliorated keratinocyte proliferation and inflammatory response, especially in IL\u201017\u2010related psoriasiform dermatitis. Furthermore, our results demonstrated that DGT prevented these pathological processes of psoriasis through suppression of STAT3 phosphorylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>DGT has great potential as a novel therapeutic agent for the treatment of psoriatic skin disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.15306","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T13:39:37Z","timestamp":1604410777000},"page":"636-653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["DGT, a novel heterocyclic diterpenoid, effectively suppresses psoriasis via inhibition of STAT3 phosphorylation"],"prefix":"10.1111","volume":"178","author":[{"given":"Gang","family":"Bian","sequence":"first","affiliation":[{"name":"Institutes of Biology and Medical Sciences Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institutes of Biology and Medical Sciences Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Xie","sequence":"additional","affiliation":[{"name":"Institutes of Biology and Medical Sciences Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanping","family":"Wang","sequence":"additional","affiliation":[{"name":"Institutes of Biology and Medical Sciences Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haimei","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Suzhou Pharmavan Co., Ltd  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunhui","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Suzhou Pharmavan Co., Ltd  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4450-8645","authenticated-orcid":false,"given":"Zijun","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiping","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Suzhou Pharmavan Co., Ltd  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunsen","family":"Li","sequence":"additional","affiliation":[{"name":"Institutes of Biology and Medical Sciences Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2009.12.010"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04911.x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14112"},{"issue":"1","key":"e_1_2_12_5_1","first-page":"S1","article-title":"The concise guide to pharmacology 2019\/20: Introduction and other protein targets","volume":"176","author":"Alexander S. 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