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The creation of metabolic profiles in faeces has received little attention until now, and reference values, especially in the context of dietary and therapeutic interventions, are missing. Exposure to xenobiotics significantly affects the physiology of the microbiome, and microbiota manipulation and short\u2010chain fatty acid administration have been proposed as treatment targets for several diseases. The aim of the present study is to give concomitant concentration ranges of faecal sterol species, bile acids and short\u2010chain fatty acids, based on a large cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Sterol species, bile acids and short\u2010chain fatty acids in human faeces from 165 study participants were quantified by LC\u2013MS\/MS. For standardization, we refer all values to dry weight of faeces. Based on the individual intestinal sterol conversion, we classified participants into low and high converters according to their coprostanol\/cholesterol ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Low converters excrete more straight\u2010chain fatty acids and bile acids than high converters; 5th and 95th percentile and median of bile acids and short\u2010chain fatty acids were calculated for both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>We give concentration ranges for 16 faecal metabolites that can serve as reference values. Patient stratification into high or low sterol converter groups is associated with significant differences in faecal metabolites with biological activities. Such stratification should then allow better assessment of faecal metabolites before therapeutic interventions.<\/jats:p><\/jats:sec><jats:sec><jats:title>LINKED ARTICLES<\/jats:title><jats:p>This article is part of a themed issue on Oxysterols, Lifelong Health and Therapeutics. To view the other articles in this section visit <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v178.16\/issuetoc\">http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v178.16\/issuetoc<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.15440","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T11:53:45Z","timestamp":1615377225000},"page":"3342-3353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Short\u2010chain fatty acids and bile acids in human faeces are associated with the intestinal cholesterol conversion status"],"prefix":"10.1111","volume":"178","author":[{"given":"Silke","family":"Matysik","sequence":"first","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine University Hospital Regensburg  Regensburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Krautbauer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine University Hospital Regensburg  Regensburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard","family":"Liebisch","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine University Hospital Regensburg  Regensburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Frieder","family":"Sch\u00f6tt","sequence":"additional","affiliation":[{"name":"Singapore Lipidomics Incubator (SLING), Life Sciences Institute National University of Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4310-9332","authenticated-orcid":false,"given":"Louise","family":"Kj\u00f8lb\u00e6k","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne","family":"Astrup","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francois","family":"Blachier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, AgroParisTech, INRAE, UMR PNCA  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Beaumont","sequence":"additional","affiliation":[{"name":"GenPhySE, Universit\u00e9 De Toulouse, INRAE, ENVT  Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max","family":"Nieuwdorp","sequence":"additional","affiliation":[{"name":"Department of Internal and Vascular Medicine Amsterdam UMC, location AMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annick","family":"Hartstra","sequence":"additional","affiliation":[{"name":"Department of Internal and Vascular Medicine Amsterdam UMC, location AMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Rampelli","sequence":"additional","affiliation":[{"name":"Unit of Microbial Ecology of Health, Department of Pharmacy and Biotechnology University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uberto","family":"Pagotto","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology and Prevention and Care of Diabetes, Sant'Orsola Hospital, Department of Medical and Surgical Sciences University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Iozzo","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology National Research Council  Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7819-12-164"},{"issue":"1","key":"e_1_2_12_3_1","first-page":"S1","article-title":"The Concise Guide to PHARMACOLOGY 2019\/20: Introduction and other protein targets","volume":"176","author":"Alexander S. 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