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The present study investigated the role of GRs and MRs in the PVN in regulating depressive and anxiety\u2010like behaviours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>To model chronic stress, rats were exposed to corticosterone treatment via drinking water for 21\u2009days, and GR antagonist RU486 and MR antagonist spironolactone, alone and combined, were directly injected in the PVN daily for the last 7\u2009days of corticosterone treatment. Behavioural tests were run on days 22 and 23. Depressive\u2010 and anxiety\u2010like behaviours were evaluated in forced swim test, sucrose preference test, novelty\u2010suppressed feeding test and social interaction test. The expression of GRs, MRs and CRF were detected by western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Rats exposed to corticosterone exhibited depressive\u2010 and anxiety\u2010like behaviours. The expression of GRs and MRs decreased, and CRF levels increased in the PVN. The intra\u2010PVN administration of RU486 increased the levels of GRs and CRF without influencing depressive\u2010 or anxiety\u2010like behaviours. The spironolactone\u2010treated group exhibited an increase in MRs without influencing GRs and CRF in the PVN and improved anxiety\u2010like behaviours. Interestingly, the intra\u2010PVN administration of RU486 and spironolactone combined restored expression of GRs, MRs and CRF and improved depressive\u2010 and anxiety\u2010like behaviours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>In this rat model of stress, the simultaneous restoration of GRs, MRs and CRF in the PVN might play an important role in the treatment of depression and anxiety.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.15511","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T03:56:00Z","timestamp":1619582160000},"page":"3696-3707","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Anti\u2010stress effects of combined block of glucocorticoid and mineralocorticoid receptors in the paraventricular nucleus of the hypothalamus"],"prefix":"10.1111","volume":"178","author":[{"given":"Hui","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u2010Ying","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010Yu","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Tong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Ling","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurhumar","family":"Kurban","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010He","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Peking University, School of Basic Medical Science  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14750"},{"key":"e_1_2_12_3_1","first-page":"407","article-title":"Goals and practicalities of immunoblotting and immunohistochemistry: A guide for submission to the British Journal of Pharmacology","volume":"3","author":"Alexander S. 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