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Previous studies in rat and guinea pig mesenteric lymphatics found that NO\u2010mediated inhibition of lymphatic contraction was prevented or reversed by the K<jats:sub>ATP<\/jats:sub> channel inhibitor, glibenclamide. We revisited this hypothesis using mouse lymphatic vessels and K<jats:sub>ATP<\/jats:sub> channel knockout mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Mouse popliteal lymphatics were isolated, and contractility was assessed using pressure myography. K<jats:sup>+<\/jats:sup> channel expression was determined by PCR analysis of FACS\u2010purified lymphatic smooth muscle cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The NO\u2010producing agonist, ACh, and the NO donor, NONOate, both produced dose\u2010dependent inhibition of spontaneous lymphatic contractions that were blocked by the soluble GC inhibitor, ODQ, or the PKG inhibitor, Rp\u20108\u2010Br\u2010PET\u2010cGMPS. Surprisingly, the inhibitory effects of both were preserved in K<jats:sub>ir<\/jats:sub>6.1<jats:sup>\u2212\/\u2212<\/jats:sup> vessels, suggesting that K<jats:sub>ATP<\/jats:sub> channels did not mediate NO\u2010induced responses. We hypothesized a role for BK channels, given their prominence in arterial smooth muscle. Indeed, BK channels were expressed in mouse lymphatic smooth muscle and NS11021 (a BK channel activator) caused dilation and reduced contraction frequency, whereas iberiotoxin and penitrem A (BK channel inhibitors) produced right\u2010ward shifts in NONOate concentration\u2013response curves.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>Inhibition of mouse lymphatic contractions by NO primarily involves activation of BK channels, rather than K<jats:sub>ATP<\/jats:sub> channels. Thus, BK channels are a potential target for therapeutic reversal of lymph pump inhibition by NO generated by immune cell activation of iNOS in chronic lymphoedema.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.15602","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T10:27:58Z","timestamp":1625221678000},"page":"4119-4136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Large\u2010conductance calcium\u2010activated K<sup>+<\/sup> channels, rather than K<sub>ATP<\/sub> channels, mediate the inhibitory effects of nitric oxide on mouse lymphatic pumping"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5095-8431","authenticated-orcid":false,"given":"Hae Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin G.","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Cell Biology &amp; Physiology Washington University  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7992-9300","authenticated-orcid":false,"given":"Michael J.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14752"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14749"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.16.7583"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP274842"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000159051"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(92)81630-2"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017095519863"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.312576"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702300"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12364"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.001bi.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14153"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP279612"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/lrb.2006.4.3"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-018-2151-y"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199804000-00013"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2015"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.002907"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14345"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2018.07.033"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.121153"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01402"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.6.C1743"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00185a021"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00316"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.280347"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.074369"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000002861"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116152108"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15178"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00392.2012"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqaa004"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2010.06.002"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0502-466"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00404"},{"key":"e_1_2_12_37_1","volume-title":"Lymphedema","author":"Olszewski W. 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