{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:19:48Z","timestamp":1788574788824,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["721432"],"award-info":[{"award-number":["721432"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background and purpose<\/jats:title>\n                    <jats:p>Corticosteroids such as triamcinolone acetonide (TAA) are potent drugs administered intra\u2010articularly as an anti\u2010inflammatory therapy to relieve pain associated with osteoarthritis (OA). However, the ability of early TAA intervention to mitigate OA progression and modulate immune cell subsets remains unclear. Here, we sought to understand the effect of early intra\u2010articular injection of TAA on OA progression, local macrophages, and peripheral blood monocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental approach<\/jats:title>\n                    <jats:p>Degenerative joint disease was induced by intra\u2010articular injection of collagenase into the knee joint of male C57BL\/6 mice. After 1\u00a0week, TAA or saline was injected intra\u2010articularly. Blood was taken throughout the study to analyse monocyte subsets. Mice were killed at days 14 and 56 post\u2010induction of collagenase\u2010induced OA (CiOA) to examine synovial macrophages and structural OA features.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>The percentage of macrophages relative to total live cells present within knee joints was increased in collagenase\u2010 compared with saline\u2010injected knees at day 14 and was not altered by TAA treatment. However, at day 56, post\u2010induction of CiOA, TAA\u2010treated knees had increased levels of macrophages compared with the knees of untreated CiOA\u2010mice. The distribution of monocyte subsets present in peripheral blood was not altered by TAA treatment during the development of CiOA. Osteophyte maturation was increased in TAA\u2010injected knees at day 56.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and implications<\/jats:title>\n                    <jats:p>Intra\u2010articular injection of TAA increases long\u2010term synovial macrophage numbers and osteophytosis. Our findings suggest that TAA accentuates the progression of osteoarthritis\u2010associated features when applied to an acutely inflamed knee.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.15780","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T21:33:21Z","timestamp":1639517601000},"page":"2771-2784","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Intra\u2010articular injection of triamcinolone acetonide sustains macrophage levels and aggravates osteophytosis during degenerative joint disease in mice"],"prefix":"10.1111","volume":"179","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2639-0724","authenticated-orcid":false,"given":"Mauricio N.","family":"Ferrao Blanco","sequence":"first","affiliation":[{"name":"Department of Orthopaedics and Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne M.","family":"Bastiaansen Jenniskens","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Kops","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athina","family":"Chavli","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Narcisi","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander M.","family":"Botter","sequence":"additional","affiliation":[{"name":"Swiss Center for Musculoskeletal Biobanking, Balgrist Campus AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter J. 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