{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:13:07Z","timestamp":1790892787586,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Varicocele is a leading cause of male infertility. Melatonin is a highly pleiotropic neurohormone. We aimed to characterize the melatonin epigenetic potential in varicocele and the involved molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Fifty\u2010two male albino rats were randomly divided into four groups (13 rats each): control (I), melatonin (II), varicocele (III) and melatonin treated varicocele (IV) groups. Left varicocele was induced by partial left renal vein ligation. Reproductive hormones, epididymal sperm functional parameters, testicular 3\/17 \u03b2\u2010hydroxysteroid dehydrogenases, antioxidant enzymes, malondialdehyde, nicotinamide adenine dinucleotide phosphate oxidase, 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine and histopathological\/Johnsen's score were evaluated. Flow cytometry and Comet were carried out to explore extent of sperm and testicular DNA damage. Testicular expression of silent information regulator 1 (SIRT1), forkhead transcription factors\u2010class O (type1) (FOXO1), tumour suppressor gene, P53, cation channels of sperm (CatSper) and steroidogenic acute regulatory protein was evaluated by western blot technique. Testicular expression of Bcl\u20102 and its associated X protein and nuclear factor kappa\u2010light\u2010chain\u2010enhancer of activated B cells were assayed by immunohistochemical staining. Testicular\n                      <jats:italic>miR\u201034a<\/jats:italic>\n                      expression was quantified by quantitative reverse transcription\u2010polymerase chain reaction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      The varicocele induced testicular histological injury, enhanced oxidative stress, P53\u2010mediated apoptosis, DNA damage and increased testicular\n                      <jats:italic>miR\u201034a<\/jats:italic>\n                      expression paralleled with down\u2010regulated SIRT1\/FOXO axis. Melatonin treatment of varicocele rats displayed antioxidant\/anti\u2010apoptotic efficacy and improved reproductive hormones axis, CatSper expression and fertility parameters. MiR\u201034a\/SIRT1\/FOXO1 epigenetic axis integrates testicular melatonin mediated intracellular transduction cascades in varicocele.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>Melatonin can be used as an adjuvant therapy to improve varicocele and its complication.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.15804","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T22:02:58Z","timestamp":1642802578000},"page":"3363-3381","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["RETRACTED: Melatonin epigenetic potential on testicular functions and fertility profile in varicocele rat model is mediated by silent information regulator 1"],"prefix":"10.1111","volume":"179","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2335-3699","authenticated-orcid":false,"given":"Rehab E.","family":"Abo El Gheit","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Tanta University  Tanta Egypt"},{"name":"Department of Physiology, Faculty of Physical therapy Al Salam University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nema A.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2345-9853","authenticated-orcid":false,"given":"Salah A.","family":"Nagla","sequence":"additional","affiliation":[{"name":"Urology Department, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rehab M.","family":"El\u2010Sayed","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Sinai University  El\u2010Arish Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4653-7597","authenticated-orcid":false,"given":"Ghada A.","family":"Badawi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Sinai University  El\u2010Arish Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa N.","family":"Emam","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Tarek","family":"Abdel Ghafar","sequence":"additional","affiliation":[{"name":"Clinical Pathology Tanta University Faculty of Medicine  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A. 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