{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T05:55:48Z","timestamp":1782021348117,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1903122"],"award-info":[{"award-number":["U1903122"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872768"],"award-info":[{"award-number":["81872768"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2022\u2010MS\u2010242"],"award-info":[{"award-number":["2022\u2010MS\u2010242"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>It is well acknowledged that tobacco\u2010derived lung carcinogens can induce lung injury and even lung cancer through a complex mechanism. MicroRNAs (MiRNAs) are differentially expressed in tobacco\u2010derived carcinogen nicotine\u2010derived nitrosamine ketone (NNK)\u2010treated A\/J mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>RNA sequencing was used to detect the level of long non\u2010coding RNAs (lncRNAs). Murine and human lung normal and cancer cells were used to evaluate the function of lncRNA XIST and miR\u2010328\u20103p in vitro, and NNK\u2010treated A\/J mice were used to test their function in vivo. In vivo levels of miR\u2010328\u20103p and lncRNA XIST were analysed, using in situ hybridization. miR\u2010328\u20103p agomir and lncRNA XIST\u2010specific siRNA were used to manipulate in vivo levels of miR\u2010328\u20103p and lncRNA XIST in A\/J mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>LncRNA XIST was up\u2010regulated in NNK\u2010induced lung injury and dominated the NNK\u2010induced ectopic miRNA expression in NNK\u2010induced lung injury both in vitro and in vivo. Either lncRNA XIST silencing or miR\u2010328\u20103p overexpression exerted opposing effects in lung normal and cancer cells regarding cell migration. LncRNA XIST down\u2010regulated miR\u2010328\u20103p levels as a miRNA sponge, and miR\u2010328\u20103p targeted the 3\u2032\u2010UTR of FZD<jats:sub>7<\/jats:sub> mRNA, which is ectopically overexpressed in lung cancer patients. Both in vivo lncRNA XIST silencing and miR\u2010328 overexpression could rescue NNK\u2010induced lung injury and aberrant overexpression of the lung cancer biomarker CK19 in NNK\u2010treated A\/J mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Our results highlight the promotive effect of lncRNA XIST in NNK\u2010induced lung injury and elucidate its post\u2010transcriptional mechanisms, indicating that targeting lncRNA XIST\/miR\u2010328\u20103p could be a potential therapeutic strategy to prevent tobacco carcinogen\u2010induced lung injury in vivo.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.16373","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T02:44:26Z","timestamp":1712630666000},"page":"2509-2527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["LncRNA XIST modulates miR\u2010328\u20103p ectopic expression in lung injury induced by tobacco\u2010specific lung carcinogen NNK both in vitro and in vivo"],"prefix":"10.1111","volume":"181","author":[{"given":"Bingxin","family":"Li","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of PIVAS Yanbian University Hospital  Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of PIVAS Yanbian University Hospital  Yanji 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