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Survivin, an anti\u2010apoptotic molecule, has been described as a regulator of cell survival. This study aims to examine the effects of YM155 treatment, a survivin selective suppressant, in maintaining neutrophil survival in vitro and in vivo experimental settings of neutrophilic inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental approach<\/jats:title><jats:p>BALB\/c mice were injected with monosodium urate (MSU) crystals and treated with YM155 (intra\u2010articularly) at the peak of inflammatory response. Leukocyte recruitment, apoptosis neutrophil and efferocytosis were determined by knee joint wash cell morphology counting and flow cytometry. Resolution interval (Ri) was quantified by neutrophil infiltration, monitoring the amplitude and duration of the inflammation. Cytokine production was measured by ELISA. Mechanical hypernociception was assessed using an electronic von Frey aesthesiometer. Efferocytosis was evaluated in zymosan\u2010induced neutrophilic peritonitis. Survivin and cleaved caspase\u20103 expression was determined in human neutrophils by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Survivin was expressed in neutrophils during MSU\u2010induced gout, and the treatment with YM155 reduced survivin expression and shortened Ri from \u223c8\u2009h observed in vehicle\u2010treated mice to \u223c5.5\u2009h, effect accompanied by increased neutrophil apoptosis and efferocytosis, both crucial for the inflammation resolution. Reduced IL\u20101\u03b2 and CXCL1 levels were also observed in periarticular tissue. YM155 reduced histopathological score and hypernociceptive response. In human neutrophils, lipopolysaccharide (LPS) increased survivin expression, whereas survivin inhibition with YM155 induced neutrophil apoptosis, with activation of caspase\u20103.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Survivin may be a promising therapeutic target to control neutrophilic inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.17375","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T04:33:50Z","timestamp":1732163630000},"page":"1206-1222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Disruption of survivin protein expression by treatment with YM155 accelerates the resolution of neutrophilic inflammation"],"prefix":"10.1111","volume":"182","author":[{"given":"D\u00e9bora de Oliveira","family":"Fernandes","sequence":"first","affiliation":[{"name":"Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Rayssa","family":"Machado","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinicius Amorim","family":"Beltrami","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Clara Paiva Menezes Dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Martins","family":"Queiroz\u2010Junior","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Priscila","family":"Vago","sequence":"additional","affiliation":[{"name":"Experimental Rheumatology, Department of Rheumatology, Radboud Institute for Molecular Life Sciences Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico Marianetti","family":"Soriani","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica, Ecologia e Evolu\u00e7\u00e3o, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-0612","authenticated-orcid":false,"given":"Fl\u00e1vio Almeida","family":"Amaral","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6944-3008","authenticated-orcid":false,"given":"Mauro Martins","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2372-2781","authenticated-orcid":false,"given":"Franciel Batista","family":"Felix","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Pinho","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-010-9196-1"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28031376"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.16181"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.16180"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032033"},{"key":"e_1_2_12_7_1","volume-title":"Methods in enzymology","author":"Amaral F. 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