{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T07:06:44Z","timestamp":1775632004120,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>\n                      Hydrogen sulphide (H\n                      <jats:sub>2<\/jats:sub>\n                      S) is synthesised endogenously through cystathionine \u03b2\u2010synthase (CBS), cystathionine \u03b3\u2010lyase (CSE) and 3\u2010mercaptopyruvate sulfurtransferase (3\u2010MST). Although exogenous H\n                      <jats:sub>2<\/jats:sub>\n                      S is known to produce vasodilatation, the vascular effect of H\n                      <jats:sub>2<\/jats:sub>\n                      S produced through 3\u2010MST is unknown. In this study, we demonstrate the effect of a novel inhibitor of 3\u2010MST, namely DPHE, and determined the effect of this compound on contractile responses in porcine coronary artery.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Synthesis of H\n                      <jats:sub>2<\/jats:sub>\n                      S through 3\u2010MST and CBS\/CSE was determined in rat liver cytosol. Effects of 3\u2010MST inhibitors DPHE, 3\u2010PAB, or I3MT\u20103, or CBS\/CSE inhibitors AOAA and propargylglycine (PPG) on contractile responses in porcine coronary arteries were determined using isolated tissue baths.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      DPHE inhibited the production of H\n                      <jats:sub>2<\/jats:sub>\n                      S from 3\u2010mercaptopyruvate (IC\n                      <jats:sub>50<\/jats:sub>\n                      \u2009~\u20098\u2009\u03bcM). The 3\u2010MST inhibitors DPHE, I3MT\u20103 and 3\u2010PAB all inhibited contractions to U46619 in porcine coronary artery segments through an endothelium\u2010independent mechanism. DPHE and I3MT\u20103 reduced the U46619 contractions in the absence of extracellular calcium and inhibited the contraction to the L\u2010type calcium channel opener BAY K8644. The combination of AOAA (100\u2009\u03bcM) and PPG (10\u2009\u03bcM) had no effect on the U46619 contractions. The inhibitory effect of the 3\u2010MST inhibitors does not appear to involve Rho kinase, ERK\u2010MAP kinase or the mitochondrial electron transport chain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Implications<\/jats:title>\n                    <jats:p>Inhibition of 3\u2010MST in coronary arteries leads to an inhibition of both calcium\u2010dependent and calcium\u2010independent contractions, whereas CBS\/CSE inhibitors had no effect on receptor\u2010mediated contractions. These data suggest that 3\u2010MST, not CBS\/CSE, regulates vascular tone in porcine coronary artery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70141","type":"journal-article","created":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T05:11:16Z","timestamp":1752901876000},"page":"5453-5468","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of 3\u2010mercaptopyruvate sulfurtransferase (3\u2010MST) inhibitors on contractile responses in porcine coronary artery"],"prefix":"10.1111","volume":"182","author":[{"given":"Maha","family":"Almaheize","sequence":"first","affiliation":[{"name":"School of Life Sciences University of Nottingham, Medical School  Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahar","family":"Alharthi","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Nottingham, Medical School  Nottingham 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