{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:56:02Z","timestamp":1773032162781,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Psoriasis is a chronic, relapsing, immune\u2010mediated inflammatory skin disease. The transient receptor potential ankyrin 1 (TRPA1) ion channel plays a protective role in the formation of psoriasiform skin reactions. Here, we investigated the pharmacological activation and blockade of TRPA1 in human skin (patho)physiology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>Six\u2010millimetre full\u2010thickness biopsies were obtained from psoriatic lesional and non\u2010lesional skin of four patients with psoriasis, and from normal skin of four healthy volunteers. Each biopsy was quartered: One segment was untreated, and the other three were cultured with vehicle (DMSO), TRPA1 agonist mustard oil (MO), or TRPA1 antagonist (HC030031), respectively. Global gene expression was measured by RNA sequencing, followed by differential expression and functional enrichment analyses, to identify TRPA1\u2010modulated genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>Pre\u2010evaluation of data with ordination assessment showed clear cluster formation according to treatments and condition of the skin. In healthy skin, TRPA1 activation down\u2010regulated genes associated with interferon signalling, antimicrobial responses, and inflammation\/oxidative stress. In lesional psoriatic skin, the genes of interleukin\u20104 (IL\u20104), IL\u201010 and IL\u201013 cytokine signalling\u2010related proteins, circadian gene expression, and senescence\u2010associated secretory phenotype (SASP) genes were down\u2010regulated by MO treatment. Antagonist treatment did not cause significant gene expression changes, supporting the previous finding that basal TRPA1 activity is low in the skin. DMSO treatment in all three conditions increased expression of several inflammatory genes, which was normalised during data analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>Exploration of the interactions between TRPA1 and identified signalling pathways may open new opportunities to target psoriasis, alleviate disease symptoms and optimise therapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70153","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T02:00:46Z","timestamp":1754532046000},"page":"5814-5825","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["TRPA1 ion channel activation context\u2010dependently regulates gene expression in normal and psoriatic human skin"],"prefix":"10.1111","volume":"182","author":[{"given":"\u00c1gnes","family":"Kem\u00e9ny","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy University of P\u00e9cs Medical School  P\u00e9cs Hungary"},{"name":"Department of Physiology and Biochemistry University of Veterinary Medicine  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szabina","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Oncodermatology University of P\u00e9cs Medical School  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00falia","family":"Szeb\u00e9nyi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Oncodermatology University of P\u00e9cs Medical School  P\u00e9cs 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