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We explored the potential anti\u2010AHR activity of selected specialised pro\u2010resolving mediators (SPMs) in IL\u201013\u2010induced AHR models, using human bronchial smooth muscle cells (BSMCs) and human isolated bronchi.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Calcium flux responses induced by histamine or LTD\n                      <jats:sub>4<\/jats:sub>\n                      were assessed in BSMCs preconditioned with IL\u201013 and SPMs for 24\u2009h. Human bronchi were isolated from lung tissue and preconditioned for 48\u2009h in the presence or absence of IL\u201013 and SPMs. Concentration\u2010response relationships for histamine and LTD\n                      <jats:sub>4<\/jats:sub>\n                      were established using myography to determine efficacy (\n                      <jats:italic>E<\/jats:italic>\n                      <jats:sub>max<\/jats:sub>\n                      ) and potency (pEC\n                      <jats:sub>50<\/jats:sub>\n                      ) following interventions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      In BSMCs, exposure to IL\u201013 increased calcium flux (\n                      <jats:italic>E<\/jats:italic>\n                      <jats:sub>max<\/jats:sub>\n                      ) triggered by histamine and LTD\n                      <jats:sub>4<\/jats:sub>\n                      . Protectin D1 (PD1) and maresin 1 (MaR1) reversed this effect, but not lipoxin A\n                      <jats:sub>4<\/jats:sub>\n                      , resolvin D2, and maresin\u2010conjugate in tissue repair 3 (MCTR3). In bronchi, IL\u201013 exposure amplified contractions to histamine and LTD\n                      <jats:sub>4<\/jats:sub>\n                      , and this enhancement was reversed by PD1 and MaR1. In contrast, PD1 and MaR1 added acutely during myography had no effect on agonist\u2010induced contractility. PD1 attenuated IL\u201013\u2013induced enhancement of airway contractions triggered by mast cell activation. CysLT\n                      <jats:sub>1<\/jats:sub>\n                      antagonism did not influence the anti\u2010hyperreactive effect of SPMs. Chemo\u2010informatics revealed structural similarities between PD1 and MaR1 that may explain the anti\u2010hyperreactive action of these two SPMs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>This new anti\u2010hyperreactive action of PD1 and MaR1 encourages further research into their potential as therapies for the treatment of airway hyperreactivity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70298","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:50:01Z","timestamp":1765932601000},"page":"2049-2060","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Protectin D1 and maresin 1 attenuate airway hyperreactivity induced by IL\u201013 in human isolated small bronchi"],"prefix":"10.1111","volume":"183","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6090-6571","authenticated-orcid":false,"given":"Willem","family":"Abma","sequence":"first","affiliation":[{"name":"Unit of Integrative Metabolomics The Institute of Environmental Medicine, Karolinska Institutet  Solna Sweden"},{"name":"Department of Respiratory Medicine and Allergy and Center for Molecular Medicine Karolinska University Hospital  Solna Sweden"},{"name":"Research and Early Development, Respiratory &amp; Immunology, BioPharmaceuticals R&amp;D, AstraZeneca  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven\u2010Erik","family":"Dahl\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine and Allergy and Center for Molecular Medicine Karolinska University Hospital  Solna Sweden"},{"name":"Clinical Lung and Allergy Research Unit, Department of Medicine Huddinge, Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig E.","family":"Wheelock","sequence":"additional","affiliation":[{"name":"Unit of Integrative Metabolomics The Institute of Environmental Medicine, Karolinska Institutet  Solna Sweden"},{"name":"Department of Respiratory Medicine and Allergy and Center for Molecular Medicine Karolinska University Hospital  Solna Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Adner","sequence":"additional","affiliation":[{"name":"Unit of Experimental Asthma and Allergy Research Institute of Environmental Medicine, Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamdoh","family":"Al\u2010Amerie","sequence":"additional","affiliation":[{"name":"Division of Thoracic Surgery, Department of Molecular Medicine and Surgery Karolinska Institutet  Stockholm Sweden"},{"name":"Thoracic Surgery, Heart and Vascular Center Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Sachs","sequence":"additional","affiliation":[{"name":"Division of Thoracic Surgery, Department of Molecular Medicine and Surgery Karolinska Institutet  Stockholm Sweden"},{"name":"Thoracic Surgery, Heart and Vascular Center Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasra","family":"Vali Jalali","sequence":"additional","affiliation":[{"name":"Thoracic Surgery, Heart and Vascular Center Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"De Maria","sequence":"additional","affiliation":[{"name":"Research and Early Development, Respiratory &amp; 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