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However, it is difficult to express insect\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 homomeric receptors to study their properties. We investigated whether the N\u2010terminal domain of each subunit could lead to functional receptors activated by acetylcholine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Human, honeybee and cockroach\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 subunits were used to synthetise chimeric receptors. Mutagenesis analysis on the N\u2010terminal domain of each\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 subunit, with confocal imaging, and electrophysiological studies, were used to build human\/insect and insect\/human homomeric constructs in\n                      <jats:italic>Xenopus<\/jats:italic>\n                      oocytes. Effects of the positive allosteric modulator, PNU\u2010120596 and inhibitor,\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2010bungarotoxin, were evaluated on each chimera. Homology modelling investigation provided a preliminary and qualitative description of the environment of the acetylcholine in the receptor's surroundings.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      Human\/insect chimeric constructs\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7H\/\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7A and\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7H\/\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7P, which include the N\u2010terminal domain of the human\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 subunit, make up the functional receptors which are activated by acetylcholine, and inhibited by\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2010bungarotoxin. In comparison, the insect\/human chimeric constructs\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7A\/\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7H and\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7P\/\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7H, which contain the N\u2010terminal domains of insect\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 subunits, are not able to form functional receptors. However, insertion of the cys\u2010loop from the human\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 subunit into the cockroach\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 subunit leads to functional receptors activated by acetylcholine, and modulated by PNU.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>\n                      This study identifies chimeric insect receptors which can be used to study the pharmacological properties of insect\n                      <jats:italic>\u03b1<\/jats:italic>\n                      7 homomeric receptors, to aid the understanding of insecticide compounds.\n                    <\/jats:p>\n                  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