{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:22:20Z","timestamp":1783012940302,"version":"3.54.6"},"reference-count":52,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471204"],"award-info":[{"award-number":["32471204"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973299"],"award-info":[{"award-number":["81973299"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104149"],"award-info":[{"award-number":["82104149"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Propofol, a widely used intravenous anaesthetic, is commonly associated with side effect of hypotension, a significant concern in clinical practice. However, the underlying mechanism of propofol\u2010induced hypotension is not fully defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Electrophysiological recordings of macroscopic and single\u2010channel currents of Ca\n                      <jats:sup>2+<\/jats:sup>\n                      activated Cl\n                      <jats:sup>\u2212<\/jats:sup>\n                      channel (\n                      <jats:styled-content>CaCC<\/jats:styled-content>\n                      ) ANO1 channels are employed.\n                      <jats:italic>Ex vivo<\/jats:italic>\n                      myography recordings of isolated mesenteric arterioles are utilized to quantify propofol\u2010induced relaxation in pre\u2010constricted blood vessels. The effects of propofol were tested\n                      <jats:italic>in vivo<\/jats:italic>\n                      by measuring changes in systolic and diastolic blood pressure following an infusion of the anaesthetic.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      Propofol inhibits macroscopic ANO1 currents expressed in HEK293T cells in a concentration\u2010dependent manner with an IC\n                      <jats:sub>50<\/jats:sub>\n                      of 39.2\u2009\u03bcM and also reduces the channel open probability without altering its single channel conductance. Four propofol analogues, 2\u2010tert\u2010butyl\u20106\u2010methylphenol, 2,6\u2010di\u2010sec\u2010butylphenol, 2,6\u2010dimethylphenol and 2,6\u2010di\u2010tert\u2010butylphenol, inhibit ANO1 currents with different potencies with IC\n                      <jats:sub>50<\/jats:sub>\n                      values variable from 59 to 3629\u2009\u03bcM.\n                      <jats:italic>Ex vivo<\/jats:italic>\n                      myography recordings demonstrate that propofol dose\u2010dependently relaxes noradrenaline\u2010preconstricted mesenteric arterioles.\n                      <jats:italic>In vivo<\/jats:italic>\n                      , intravenous infusion of propofol significantly reduces both systolic and diastolic blood pressure. Conversely, propofol\u2010induced hypotension is reversed by co\u2010administration of ANO1 channel opener Eact.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Implications<\/jats:title>\n                    <jats:p>Propofol causes arterial vasorelaxation and ANO1 inhibition by propofol contributes to its blood pressure\u2010lowering effect. This mechanism may explain the hypotension frequently encountered during clinical use of propofol, providing an insight into strategies to mitigate this side effect.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70467","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:05:07Z","timestamp":1777511107000},"page":"4416-4429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel role of propofol and its analogues in arterial vasorelaxation and blood pressure reduction via inhibition of calcium activated chloride channel ANO1"],"prefix":"10.1111","volume":"183","author":[{"given":"Wei","family":"Lv","sequence":"first","affiliation":[{"name":"Departments of Pharmacology Qingdao University Medical College  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoya","family":"Rong","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology Qingdao University Medical College  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yepeng","family":"Luan","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry, School of Pharmacy Qingdao University Medical College  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Tang","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry, School of Pharmacy Qingdao University Medical College  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingdi","family":"Sun","sequence":"additional","affiliation":[{"name":"Nhwa Institute of Pharmaceutical Research Jiangsu Nhwa Pharmaceuticals  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Miao","sequence":"additional","affiliation":[{"name":"Nhwa Institute of Pharmaceutical Research Jiangsu Nhwa Pharmaceuticals  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanbiao","family":"Mei","sequence":"additional","affiliation":[{"name":"Nhwa Institute of Pharmaceutical Research Jiangsu Nhwa Pharmaceuticals  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangqi","family":"Xu","sequence":"additional","affiliation":[{"name":"Nhwa Institute of Pharmaceutical Research Jiangsu Nhwa Pharmaceuticals  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Dong","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology Qingdao University Medical College  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yani","family":"Liu","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology Qingdao University Medical College  Qingdao China"},{"name":"Institute of Innovative Drugs Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7023-9998","authenticated-orcid":false,"given":"KeWei","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology Qingdao University Medical College  Qingdao China"},{"name":"Institute of Innovative Drugs Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.70231"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2022.06.006"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0000000000000967"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15454"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2018-0336"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/85.6.901"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.03.025"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1992.72.1.231"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011980432646"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.17441"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jvca.2018.12.015"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199205000-00010"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/367607a0"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15322"},{"key":"e_1_2_12_16_1","first-page":"2252","article-title":"Blood pressure regulation: Basic concepts","volume":"40","author":"Guyton A. C.","year":"1981","journal-title":"Federation Proceedings"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.115.018526"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI70025"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bja.2018.12.025"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(93)90337-w"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.35.1.173"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.115320"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/81.5.766"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3565(24)29544-6"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1441-2772(23)01503-X"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aes069"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202313460"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15178"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1572-5"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181b799c1"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-0105.177703"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-191627"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"e_1_2_12_34_1","first-page":"1191","article-title":"Propofol\u2010associated dilation of rat distal coronary arteries is mediated by multiple substances, including endothelium\u2010derived nitric oxide","volume":"81","author":"Park K. W.","year":"1995","journal-title":"Anesthesia and Analgesia"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15193"},{"key":"e_1_2_12_36_1","volume-title":"StatPearls Treasure Island [FL] ineligible companies","author":"Rehman S.","year":"2025"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199701000-00010"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e31823d0c36"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199403000-00009"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199304000-00008"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aeq190"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934838"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2004.027441"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867003375335"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.avsg.2019.04.005"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2018.6527"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.02.020"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13797"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-53491-0.00008-0"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07313"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1340"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10112760"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2021.11.001"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.70467","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bph.70467","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.70467","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:50:41Z","timestamp":1783011041000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.70467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":52,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/bph.70467"],"URL":"https:\/\/doi.org\/10.1111\/bph.70467","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]},"assertion":[{"value":"2025-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}