{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:57:07Z","timestamp":1792205827667,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20814"],"award-info":[{"award-number":["U24A20814"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>\n                      Atherosclerosis is a chronic inflammatory disease. Targeting inflammatory pathways provides a promising avenue to treat atherosclerosis. Osthole (OS), isolated from the\n                      <jats:italic>Cnidium<\/jats:italic>\n                      plant, has been reported diverse pharmacological activities, including anti\u2010cancer, anti\u2010oxidant, neuroprotective, anti\u2010osteoporosis and anti\u2010inflammatory effects. However, the role on atherosclerosis and molecular targets of osthole remains unclear. We aimed to explore the anti\u2010atherosclerosis role of osthole and investigate the underlying molecular mechanism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Mouse primary peritoneal macrophages (MPMs) were isolated and treated with oxLDL\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Protein microarray and molecular docking were used to identify the target\/s of osthole.\n                      <jats:italic>ApoE<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice were fed with a high\u2010fat diet (HFD) for 8\u00a0weeks to induce atherosclerosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      Osthole inhibited the inflammatory factor secretion induced by oxLDL and then reduced oxLDL uptake in MPMs.\n                      <jats:italic>In vivo<\/jats:italic>\n                      , osthole administration alleviated atherosclerotic plaque formation and inflammatory response in HFD\u2010fed\n                      <jats:italic>ApoE<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice. Mechanistically, protein microarray incubated with biotin\u2010labelled osthole identified doublecortin like kinase 1 (DCLK1\/DCAMKL1) as the top\u2010ranked binding protein of osthole. Osthole directly bound to DCLK1 at I396 and L518 sites, inhibited the phosphorylation of DCLK1, and then prevented its interaction with inhibitor of nuclear factor kappa B kinase subunit beta (IKK\u03b2). Through targeting DCLK1, osthole suppressed NF\u2010\u03baB pathway activation and inflammatory responses in both MPMs and aortic lesions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>Taken together, our findings show the therapeutic potential of osthole against inflammatory atherosclerosis and establish a foundation for targeting DCLK1 therapy in atherosclerosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70475","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:56:42Z","timestamp":1777607802000},"page":"4576-4591","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Osthole targets doublecortin like kinase 1 (DCLK1\/DCAMKL1) in macrophages to inhibit NF\u2010\u03baB\u2010mediated inflammation and alleviate atherosclerosis"],"prefix":"10.1111","volume":"183","author":[{"given":"Sirui","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Zhejiang Provincial Key Laboratory of Liver Diseases The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"},{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Zhejiang Provincial Key Laboratory of Liver Diseases The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"},{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Guan","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yudie","family":"Yang","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Chen","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Xu","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Zhejiang Provincial Key Laboratory of Liver Diseases The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1603-3223","authenticated-orcid":false,"given":"Guang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Zhejiang Provincial Key Laboratory of Liver Diseases The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"},{"name":"Chemical Biology Research Center, School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou Zhejiang China"},{"name":"School of Pharmaceutical Sciences Hangzhou Medical College  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-07166-8"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.70234"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.70233"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14112"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2021.02.001"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102706"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100191"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.17441"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2023.104118"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-024-01039-5"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.107.149179"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12051023"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.115.306301"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-1925-z"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202217198"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-022-00928-0"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.09.019"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2025.107833"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154640"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4934592"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112131"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110139"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2017.06.025"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15178"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-023-01147-8"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology14060588"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001303"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.70037"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02631-y"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehz542"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2009.12.017"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi026913i"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110066"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2023.117487"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00198-1"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.115.306256"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2025.1671008"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.1730"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacl.2017.06.015"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.115453"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05960-4"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c09749"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.945627"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.115848"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2023.122294"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.119.315861"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557520666200709175948"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.975367"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2025.108864"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.70475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bph.70475","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.70475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T08:25:25Z","timestamp":1783671925000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.70475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":50,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/bph.70475"],"URL":"https:\/\/doi.org\/10.1111\/bph.70475","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]},"assertion":[{"value":"2026-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}