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Here, we investigated the precise intracellular signalling pathways leading to vasodilation in small coronary arteries.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      This study used\n                      <jats:italic>ex vivo<\/jats:italic>\n                      myography and intracellular recording techniques to investigate \u03b1\u2010CGRP\u2010induced vasorelaxation and vascular smooth muscle cell (VSMC) hyperpolarization in myogenically active rat isolated coronary arteries.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      CGRP\u2010induced vasorelaxation was not dependent on the endothelium, but relied heavily on K\n                      <jats:sup>+<\/jats:sup>\n                      channel activation. Immunohistochemistry indicated K\n                      <jats:sub>V<\/jats:sub>\n                      7 and BK\n                      <jats:sub>Ca<\/jats:sub>\n                      channel expression in VSMC and endothelial cells. A combination of the K\n                      <jats:sub>V<\/jats:sub>\n                      7 channel inhibitor, linopirdine and the BK\n                      <jats:sub>Ca<\/jats:sub>\n                      channel inhibitor, paxilline, significantly attenuated CGRP\u2010induced vasorelaxation in endothelium\u2010intact or denuded arteries. Electrophysiology confirmed that CGRP caused hyperpolarization and showed this was prevented by linopirdine and paxilline, also demonstrating a role for K\n                      <jats:sub>V<\/jats:sub>\n                      7 and BK\n                      <jats:sub>Ca<\/jats:sub>\n                      channels in suppressing depolarizing smooth muscle spikes. These spikes were also suppressed by NO\u2022 and HNO donors, resulting in hyperpolarization. G\u03b2\u03b3 subunit inhibition with gallein markedly right\u2010shifted CGRP\u2010induced vasorelaxation in both endothelium\u2010intact and denuded coronary arteries.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>\n                      \u03b1\u2010CGRP stimulates robust vasorelaxation in the coronary microvasculature that relies on G\u03b2\u03b3 subunit\u2010activated VSMC hyperpolarization involving K\n                      <jats:sub>V<\/jats:sub>\n                      7 and BK\n                      <jats:sub>Ca<\/jats:sub>\n                      channels. These data enhance understanding of coronary microvascular physiology and inform the design of future therapeutic strategies targeting coronary vascular dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70492","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T03:56:22Z","timestamp":1779249382000},"page":"5018-5032","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["G\u03b2\u03b3 subunit activation of K\n                    <sub>V<\/sub>\n                    7 and BK\n                    <sub>Ca<\/sub>\n                    channels underlies calcitonin gene\u2010related peptide vasorelaxation in myogenic rat coronary resistance arteries"],"prefix":"10.1111","volume":"183","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8667-3032","authenticated-orcid":false,"given":"Lucy 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