{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:47:17Z","timestamp":1785775637859,"version":"3.56.0"},"reference-count":49,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Fucosyltransferase 8 (FUT8)\u2010mediated core fucosylation (CF) has been confirmed to regulate multiple disease progression. However, its role in myocardial ischaemia\/reperfusion (I\/R) injury are unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>The mouse model of myocardial I\/R injury was constructed, injecting 2\u2010fluorofucose (2FF) to explore the effect of CF inhibitor on myocardial injury. Infarct size was analysed by TTC staining. Myocardial injury was assessed by H&amp;E staining, Masson staining, myocardial enzyme level detection and cardiac function evaluation. CF level was assessed by lectin blotting and immunofluorescence. The levels of macrophage markers, JAK2\/STAT3\u2010related markers, FUT8 and Kr\u00fcppel\u2010like factor 6 (KLF6) were examined by western blot or qRT\u2010PCR. RAW264.7 cells were induced with hypoxia\/reoxygenation (H\/R). Flow cytometry measured macrophage surface markers. The effect of FUT8 on CF level of KLF6 was assessed by immunoprecipitation assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>CF level was up\u2010regulated in myocardial I\/R injury mice, and its inhibitor 2FF alleviated myocardial I\/R injury by repressing macrophage M1 polarization. Furthermore, FUT8 increased expression in myocardial I\/R injury patients and mice, and its down\u2010regulation suppressed macrophage M1 polarization in H\/R\u2010induced RAW264.7 cells. FUT8 enhanced the CF level of KLF6 to activate the JAK2\/STAT3 pathway, and FUT8 knockdown reversed the promoting effect of KLF6 overexpression on macrophage M1 polarization. Moreover, FUT8 knockout alleviated myocardial I\/R injury by inactivating the KLF6\/JAK2\/STAT3 pathway and inhibiting macrophage M1 polarization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>FUT8\u2010mediated CF of KLF6 promoted macrophage M1 polarization to aggravate myocardial I\/R injury by activating JAK2\/STAT3 pathway, providing a novel treatment of myocardial I\/R injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70494","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:30:44Z","timestamp":1780630244000},"page":"5284-5299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fucosyltransferase 8\u2010mediated core fucosylation of kr\u00fcppel\u2010like factor 6 exacerbates myocardial ischaemia\/reperfusion injury via promoting macrophage M1 polarization"],"prefix":"10.1111","volume":"183","author":[{"given":"Haiyan","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of University of South China  Hengyang City Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of University of South China  Hengyang City Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Yiyang Central Hospital  Yiyang City Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binlan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Respiratory and Intensive Care Unit (Ricu) The First Affiliated Hospital of University of South China  Hengyang 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