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We aimed to develop an animal model to study these phenomena.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>Naive and anti\u2010polyethylene (PEG) antibody\u2010sensitised pigs were injected with Comirnaty i.v. Responses were evaluated by haemodynamic monitoring, haematological changes and analysis of inflammatory markers. Inflammatory cytokine gene up\u2010regulation was tested by RT\u2010qPCR at 6\u2009h and at 2 and 6\u2009weeks after vaccination. Vaccine mRNA uptake was followed, and histopathology and spike protein immunohistochemistry were performed in selected organs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>Cardiopulmonary distress of varying severity developed within minutes after each Comirnaty injection. Vaccine mRNA accumulation peaked in peripheral mononuclear cells (PBMC) within 15\u2009min and then began to decline within minutes. After 6 h vaccine mRNA was high in liver and spleen, moderate in kidney, lymph nodes, and heart, and low in brain. At the same time expression of mRNA for proinflammatory cytokines (IL\u20101RA, CXCL10, TNF\u2010\u03b1, CCL2) varied in different organs and paralleled with vaccine mRNA uptake, suggesting a close association. Histological abnormalities and spike protein immunopositivity were noted in kidney, heart and brain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Implications<\/jats:title>\n                    <jats:p>This porcine model revealed systemic anaphylactic reactivity and multiorgan vaccine mRNA uptake following i.v. injection of mRNA\u2010LNPs, with organ\u2010specific inflammatory response at 6\u2009h and thereafter. These findings provide mechanistic insights into rare vaccine\u2010related acute AEs and suggest future efforts to improve the safety of the mRNA\u2010LNP platform.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70563","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T14:05:09Z","timestamp":1785074709000},"page":"6225-6239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acute anaphylactic and multiorgan inflammatory effects of Comirnaty in pigs: evidence of spike protein mRNA transfection and paralleling inflammatory cytokine upregulation"],"prefix":"10.1111","volume":"183","author":[{"given":"L\u00e1szl\u00f3","family":"D\u00e9zsi","sequence":"first","affiliation":[{"name":"Nanomedicine Research and Education Centre, Institute of Clinical Pathophysiology Semmelweis University  Budapest Hungary"},{"name":"Institute of Clinical Pathophysiology Semmelweis 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