{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:55:17Z","timestamp":1789905317149,"version":"4.0.1"},"reference-count":70,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Ischaemic stroke is characterised by acute cerebrovascular occlusion, blood\u2013brain barrier (BBB) breakdown and a narrow therapeutic window for recovery. Its treatment is a clinical challenge due to the risk of reperfusion injury and the limited efficacy of thrombolytic therapies; therefore, novel therapeutic approaches are needed. Histone deacetylase inhibitors (HDACi) have emerged as neuroprotective agents in stroke models, but their effect on preserving BBB integrity is unexplored. Our aim was to investigate the effects of the HDACi, suberoylanilide hydroxamic acid (SAHA), on BBB changes in a cell culture model of ischaemic stroke.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>The effects of SAHA were tested on a human BBB co\u2010culture model following a 6\u2010h oxygen\u2013glucose deprivation (OGD) under normoxia and during a 24 h reoxygenation (OGD\/R).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>SAHA treatment ameliorated the OGD\/R\u2010induced loss of BBB integrity, as shown by an increase in transendothelial electrical resistance and reduced BBB permeability. The expression of genes involved in cell proliferation decreased, whereas an increase was measured for basement membrane protein, glycocalyx\u2010synthesis enzyme and Wnt signalling\u2013related genes. SAHA treatment elevated the claudin\u20105 protein expression and a metabolic shift from glycolysis to aerobic respiration was observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Implications<\/jats:title>\n                    <jats:p>Our results suggest that SAHA could be a potential adjunctive therapeutic drug for the treatment of ischaemia\u2013reperfusion injury via BBB protection. Because SAHA has already been approved for human use as the anticancer drug vorinostat, its repurposing to restore BBB functions and prevent poststroke damages may be greatly facilitated.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70568","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T05:22:33Z","timestamp":1783920153000},"page":"5791-5812","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The histone deacetylase inhibitor, suberoylanilide hydroxamic acid, restores blood\u2013brain barrier integrity in a human stem cell\u2010based model of ischaemic stroke"],"prefix":"10.1111","volume":"183","author":[{"given":"Anik\u00f3","family":"Szecsk\u00f3","sequence":"first","affiliation":[{"name":"Biological Barriers Research Group, Institute of Biophysics HUN\u2010REN Biological Research Centre  Szeged Hungary"},{"name":"Doctoral School of Biology University of 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