{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:04:17Z","timestamp":1790647457840,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82470799"],"award-info":[{"award-number":["82470799"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022QH324"],"award-info":[{"award-number":["ZR2022QH324"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Phloroglucinol is used to treat renal colic, especially when non\u2010steroidal anti\u2010inflammatory drugs are contraindicated. This study aimed to clarify its mechanism by examining effects on human ureteral contractility and underlying signalling pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Urinary levels of neurokinin A (NKA), substance P (SP), 5\u2010hydroxytryptamine (5\u2010HT) and PGE\n                      <jats:sub>2<\/jats:sub>\n                      were measured in patients after acute renal colic. Human ureteral tissues from organ donors were studied in organ baths and myosin light chain (MLC) phosphorylation in human ureteral smooth muscle cells (hUSMCs) was assessed as a marker of contractile activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      Urinary NKA, SP and 5\u2010HT but not PGE\n                      <jats:sub>2<\/jats:sub>\n                      were significantly increased in patients with colic. Phloroglucinol concentration\u2010dependently inhibited rises in the basal tone during spasmic contractions evoked by high concentrations of KCl, NKA and 5\u2010HT. Phloroglucinol also attenuated the frequency of spontaneous or low concentrations of NKA\u2010evoked (30\u2013100\u2009nM) and 5\u2010HT\u2010evoked (3\u201310\u00a0\u03bcM) phasic contractions. The above inhibitory effects of phloroglucinol were significantly attenuated in the presence of SQ22536 (adenylate cyclase inhibitor) or H89 (PKA inhibitor). Although phloroglucinol attenuated EFS\u2010evoked (neurogenic) contractions, this effect was markedly weaker than that observed for myogenic contractions. Phloroglucinol increased intracellular levels of cAMP and PKA in hUSMCs, and reduced the enhanced MLC phosphorylation evoked by NKA (10\u00a0\u03bcM) and 5\u2010HT (100\u2009\u03bcM).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Implications<\/jats:title>\n                    <jats:p>These findings support the clinical use of phloroglucinol in treating renal colic, inhibiting ureteral spasm contractions via the activation of cAMP\u2010PKA signalling and provides a rationale for its potential utility in facilitating stone expulsion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.70672","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:47:17Z","timestamp":1790646437000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phloroglucinol inhibits human ureteral 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