{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:59:22Z","timestamp":1769576362822,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T00:00:00Z","timestamp":1372118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>The marine snail peptide ziconotide (\u03c9\u2010conotoxin <jats:styled-content style=\"fixed-case\">MVIIA<\/jats:styled-content>) is used as an analgesic in cancer patients refractory to opioids, but may induce severe adverse effects. Animal venoms represent a rich source of novel drugs, so we investigated the analgesic effects and the side\u2010effects of spider peptide <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>h\u03b11\u03b2 in a model of cancer pain in mice with or without tolerance to morphine analgesia. Cancer pain was induced by the inoculation of melanoma <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>16\u2010<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>10 cells into the hind paw of <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice. After 14\u00a0days, painful hypersensitivity was detected and <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>h\u03b11\u03b2 or \u03c9\u2010conotoxin <jats:styled-content style=\"fixed-case\">MVIIA<\/jats:styled-content> (10\u2013100\u00a0pmol\/site) was intrathecally injected to evaluate the development of antinociception and side\u2010effects in control and morphine\u2010tolerant mice. The treatment with <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>h\u03b11\u03b2 or \u03c9\u2010conotoxin <jats:styled-content style=\"fixed-case\">MVIIA<\/jats:styled-content> fully reversed cancer\u2010related painful hypersensitivity, with long\u2010lasting results, at effective doses 50% of 48 (32\u201372) or 33 (21\u201353)\u00a0pmol\/site, respectively. Ph\u03b11\u03b2 produced only mild adverse effects, whereas \u03c9\u2010conotoxin <jats:styled-content style=\"fixed-case\">MVIIA<\/jats:styled-content> induced dose\u2010related side\u2010effects in mice at analgesic doses (estimated toxic dose 50% of 30\u00a0pmol\/site). In addition, we observed that <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>h\u03b11\u03b2 was capable of controlling cancer\u2010related pain even in mice tolerant to morphine antinociception (100% of inhibition) and was able to partially restore morphine analgesia in such animals (56\u00a0\u00b1\u00a05% of inhibition). In this study, <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>h\u03b11\u03b2 was as efficacious as \u03c9\u2010conotoxin <jats:styled-content style=\"fixed-case\">MVIIA<\/jats:styled-content> in inducing analgesia in a model of cancer pain without producing severe adverse effects or losing efficacy in opioid\u2010tolerant mice, indicating that <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>h\u03b11\u03b2 has a good profile for the treatment of cancer pain in patients.<\/jats:p>","DOI":"10.1111\/cas.12209","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T15:21:04Z","timestamp":1369840864000},"page":"1226-1230","source":"Crossref","is-referenced-by-count":57,"title":["Spider peptide Ph\u03b11\u03b2 induces analgesic effect in a model of cancer pain"],"prefix":"10.1111","volume":"104","author":[{"given":"Flavia Karine","family":"Rigo","sequence":"first","affiliation":[{"name":"Graduate Program in Biochemistry and 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