{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:37:56Z","timestamp":1788579476846,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T00:00:00Z","timestamp":1375747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>\n                    High\u2010resolution matrix\u2010assisted laser desorption\/ionization imaging mass spectrometry (\n                    <jats:styled-content style=\"fixed-case\">MALDI IMS<\/jats:styled-content>\n                    ) is an emerging application for lipid research that provides a comprehensive and detailed spatial distribution of ionized molecules. Recent lipidomic approach has identified several phospholipids and phosphatidylinositols (\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s) are accumulated in breast cancer tissues and are therefore novel biomarker candidates. Because their distribution and significance remain unclear, we investigated the precise spatial distribution of\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s in human breast cancer tissues using high\u2010resolution\n                    <jats:styled-content style=\"fixed-case\">MALDI IMS<\/jats:styled-content>\n                    . We evaluated tissues from nine human breast cancers and one normal mammary gland by negative ion\n                    <jats:styled-content style=\"fixed-case\">MALDI IMS<\/jats:styled-content>\n                    at a resolution of 10\u00a0\u03bcm. We detected 10\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s with different fatty acid compositions, and their proportions were remarkably variable in the malignant epithelial regions. High\u2010resolution imaging enabled us to discriminate cancer cell clusters from the adjacent stromal tissue within epithelial regions; moreover, this technique revealed that several\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s were specifically localized to cancer cell clusters. These\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s were heterogeneously distributed within cancer cell clusters, allowing us to identify two different populations of cancer cells that predominantly expressed either\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    (18:0\/18:1) or\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    (18:0\/20:3). Tracing the expression level of\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s during cancer cell progression suggested that the latter population is associated with the invasion. Our study documents a novel model for phospholipid analysis of breast cancer tissues by using high\u2010resolution\n                    <jats:styled-content style=\"fixed-case\">MALDI IMS<\/jats:styled-content>\n                    and identifies candidate\n                    <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\n                    s that can describe a specific phenotype of cancer cells.\n                  <\/jats:p>","DOI":"10.1111\/cas.12229","type":"journal-article","created":{"date-parts":[[2013,7,10]],"date-time":"2013-07-10T03:16:47Z","timestamp":1373426207000},"page":"1372-1379","source":"Crossref","is-referenced-by-count":70,"title":["High\u2010resolution imaging mass spectrometry reveals detailed spatial distribution of phosphatidylinositols in human breast cancer"],"prefix":"10.1111","volume":"104","author":[{"given":"Masahiro","family":"Kawashima","sequence":"first","affiliation":[{"name":"Department of Breast Surgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Koichi Tanaka Laboratory of Advanced Science and Technology Shimadzu Corporation Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Kawaguchi\u2010Sakita","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Ueno","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Mikami","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuya","family":"Terasawa","sequence":"additional","affiliation":[{"name":"World\u2010Leading Drug Discovery Research Center Graduate School of Pharmaceutical Sciences Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taka\u2010Aki","family":"Sato","sequence":"additional","affiliation":[{"name":"Koichi Tanaka Laboratory of Advanced Science and Technology Shimadzu Corporation Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Koichi Tanaka Laboratory of Advanced Science and Technology Shimadzu Corporation Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuharu","family":"Shimizu","sequence":"additional","affiliation":[{"name":"World\u2010Leading Drug Discovery Research Center Graduate School of Pharmaceutical Sciences Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Toi","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Graduate School of Medicine Kyoto University Kyoto 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