{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:08:42Z","timestamp":1787314122006,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2013,11]]},"abstract":"\n Recent progression in the understanding of stem cell biology has greatly facilitated the identification and characterization of cancer stem cells (\n CSC<\/jats:styled-content>\n s). Moreover, evidence has accumulated indicating that conventional cancer treatments are potentially ineffective against\n CSCs<\/jats:styled-content>\n . Histone deacetylase inhibitors (\n HDAC<\/jats:styled-content>\n i) have multiple biologic effects consequent to alterations in the patterns of acetylation of histones and are a promising new group of anticancer agents. In this study, we investigated the effects of two\n HDAC<\/jats:styled-content>\n i, suberoylanilide hydroxamic acid (\n SAHA<\/jats:styled-content>\n ) and trichostatin A (\n TSA<\/jats:styled-content>\n ), on two\n CD<\/jats:styled-content>\n 44+ cancer stem\u2010like cell lines from squamous cell carcinoma of the head and neck (\n SCCHN<\/jats:styled-content>\n ) cultured in serum\u2010free medium containing epidermal growth factor and basic fibroblast growth factor. Histone deacetylase inhibitors inhibited the growth of\n SCCHN<\/jats:styled-content>\n cell lines in a dose\u2010dependent manner as measured by\n MTS<\/jats:styled-content>\n assays. Moreover,\n HDAC<\/jats:styled-content>\n i induced cell cycle arrest and apoptosis in these\n SCCHN<\/jats:styled-content>\n cell lines. Interestingly, the expression of cancer stem cell markers,\n CD<\/jats:styled-content>\n 44 and\n ABCG<\/jats:styled-content>\n 2, on\n SCCHN<\/jats:styled-content>\n cell lines was decreased by\n HDAC<\/jats:styled-content>\n i treatment. In addition,\n HDAC<\/jats:styled-content>\n i decreased\n mRNA<\/jats:styled-content>\n expression levels of stemness\u2010related genes and suppressed the epithelial\u2010mesencymal transition phenotype of\n CSC<\/jats:styled-content>\n s. As expected, the combination of\n HDACi<\/jats:styled-content>\n and chemotherapeutic agents, including cisplatin and docetaxel, had a synergistic effect on\n SCCHN<\/jats:styled-content>\n cell lines. Taken together, our data indicate that\n HDAC<\/jats:styled-content>\n i not only inhibit the growth of\n SCCHN<\/jats:styled-content>\n cell lines by inducing apoptosis and cell cycle arrest, but also alter the cancer stem cell phenotype in\n SCCHN<\/jats:styled-content>\n , raising the possibility that\n HDAC<\/jats:styled-content>\n i may have therapeutic potential for cancer stem cells of\n SCCHN<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/cas.12271","type":"journal-article","created":{"date-parts":[[2013,8,30]],"date-time":"2013-08-30T07:46:51Z","timestamp":1377848811000},"page":"1468-1475","source":"Crossref","is-referenced-by-count":52,"title":["Alteration of cancer stem cell\u2010like phenotype by histone deacetylase inhibitors in squamous cell carcinoma of the head and neck"],"prefix":"10.1111","volume":"104","author":[{"given":"Kazuaki","family":"Chikamatsu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Gunma University Graduate School of Medicine Maebashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Faculty of Medicine University of Yamanashi Japan"},{"name":"Department of Biochemistry Interdisciplinary Graduate School of Medicine and Engineering University of Yamanashi Chuo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Gunma University Graduate School of Medicine Maebashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Sakakura","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Gunma University Graduate School of Medicine Maebashi 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