{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:53:56Z","timestamp":1790146436615,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,3,30]],"date-time":"2014-03-30T00:00:00Z","timestamp":1396137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>We aimed to reveal the prevalence and pattern of human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) infection and <jats:italic>p53<\/jats:italic> mutations among Japanese head and neck squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>) patients in relation to clinicopathological parameters. Human papillomavirus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and <jats:italic>p53<\/jats:italic> mutations were examined in 493 <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>s and its subset of 283 <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>s. Oropharyngeal carcinoma was more frequently <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive than non\u2010oropharyngeal carcinoma (34.4% <jats:italic>vs<\/jats:italic> 3.6%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), and <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16 accounted for 91.1% of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive tumors. In oropharyngeal carcinoma, which showed an increasing trend of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> prevalence over time (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection was inversely correlated with tobacco smoking, alcohol drinking, <jats:italic>p53<\/jats:italic> mutations, and a disruptive mutation (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003<jats:italic>,<\/jats:italic> &lt;0.001, &lt;0.001, and &lt;0.001, respectively). The prevalence of <jats:italic>p53<\/jats:italic> mutations differed significantly between virus\u2010unrelated <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content> and virus\u2010related <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content> consisting of nasopharyngeal and <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive oropharyngeal carcinomas (48.3% <jats:italic>vs<\/jats:italic> 7.1%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Although <jats:italic>p53<\/jats:italic> mutations were associated with tobacco smoking and alcohol drinking, this association disappeared in virus\u2010unrelated <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>. A disruptive mutation was never found in virus\u2010related <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>, whereas it was independently associated with primary site, such as the oropharynx and hypopharynx (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01 and 0.03, respectively), in virus\u2010unrelated <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>. Moreover, in virus\u2010unrelated <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>, G:C to T:A transversions were more frequent in ever\u2010smokers than in never\u2010smokers (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04), whereas G:C to A:T transitions at CpG sites were less frequent in ever\u2010smokers than in never\u2010smokers (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04). In conclusion, <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content> is etiologically classified into virus\u2010related and virus\u2010unrelated subgroups. In virus\u2010related <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>,<jats:italic> p53<\/jats:italic> mutations are uncommon with the absence of a disruptive mutation, whereas in virus\u2010unrelated <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>,<jats:italic> p53<\/jats:italic> mutations are common, and disruptive mutagenesis of <jats:italic>p53<\/jats:italic> is related with oropharyngeal and hypopharyngeal carcinoma.<\/jats:p>","DOI":"10.1111\/cas.12369","type":"journal-article","created":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T08:29:56Z","timestamp":1392280196000},"page":"409-417","source":"Crossref","is-referenced-by-count":89,"title":["Human papillomavirus and <i>p53<\/i> mutations in head and neck squamous cell carcinoma among Japanese population"],"prefix":"10.1111","volume":"105","author":[{"given":"Hiromi","family":"Maruyama","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshimichi","family":"Yasui","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Ishikawa\u2010Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Radiation Biology and Medical Genetics Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiichi","family":"Morii","sequence":"additional","affiliation":[{"name":"Department of Pathology Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshifumi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Yoshii","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukinori","family":"Takenaka","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Nakahara","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Osaka University Faculty of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Todo","sequence":"additional","affiliation":[{"name":"Department of Radiation Biology and Medical Genetics Osaka 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