{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T11:38:47Z","timestamp":1789558727818,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"name":"National Cancer Center Research and Development Fund","award":["23-A-12 and 23-K-18"],"award-info":[{"award-number":["23-A-12 and 23-K-18"]}]},{"DOI":"10.13039\/501100007077","name":"the Advanced research for medical products Mining Programme of the National Institute of Biomedical Innovation (NIBIO)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007077","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Foundation for the Promotion of Cancer Research, 3rd-Term Comprehensive 10-Year Strategy for Cancer Control"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>Patients with mediastinal lymph node metastasis (N2) in squamous cell carcinoma (Sq<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>) of the lung have poor prognosis after surgical resection of the primary tumor. The aim of this study was to clarify predictive factors of the recurrence of pathological lung Sq<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content> with N2 focusing on the biological characteristics of both cancer cells and cancer\u2010associated fibroblasts (<jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>s) in primary and metastatic lymph node tumors. We selected 64 patients with pathological primary lung N2 Sq<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content> who underwent surgical complete resection and investigated the expressions of four epithelial\u2013mesenchymal transition\u2010related markers (caveolin, clusterin, E\u2010cadherin, <jats:styled-content style=\"fixed-case\">ZEB<\/jats:styled-content>2), three cancer stem cell\u2010related markers (<jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44 variant6, podoplanin) of cancer cells, and four markers of <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>s (caveolin, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>90, clusterin, podoplanin) in both primary and matched metastatic lymph node tumors in the N2 area. In the primary tumors, the expressions of all the examined molecules were not related to recurrence. However, in the metastatic lymph node tumors, high clusterin and <jats:styled-content style=\"fixed-case\">ZEB<\/jats:styled-content>2 expressions in the cancer cells and high podoplanin expression in the <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>s were significantly correlated with recurrence (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.03, 0.04, and 0.007, respectively). In a multivariate analysis, only podoplanin expression in the <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>s in metastatic lymph node tumors was identified as a significantly independent predictive factor of recurrence (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.03). Our study indicated that the immunophenotypes of both cancer cells and <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>s in metastatic lymph node tumors, but not primary tumors, provide useful information for predicting the recurrence of pathological N2 lung Sq<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/cas.12434","type":"journal-article","created":{"date-parts":[[2014,5,11]],"date-time":"2014-05-11T10:24:35Z","timestamp":1399803875000},"page":"905-911","source":"Crossref","is-referenced-by-count":21,"title":["Immunophenotypic features of metastatic lymph node tumors to predict recurrence in <scp>N<\/scp>2 lung squamous cell carcinoma"],"prefix":"10.1111","volume":"105","author":[{"given":"Rie","family":"Matsuwaki","sequence":"first","affiliation":[{"name":"Division of Pathology, Research Center for Innovative Oncology National Cancer Center Hospital East Kashiwa Japan"},{"name":"Division of Thoracic Surgery 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