{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:25:00Z","timestamp":1791188700761,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T00:00:00Z","timestamp":1434499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Medicine Leading Talents of Jiangsu Province","award":["LJ201131"],"award-info":[{"award-number":["LJ201131"]}]},{"name":"the grant from Jiangsu Health Department","award":["H201325"],"award-info":[{"award-number":["H201325"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["21475063"],"award-info":[{"award-number":["21475063"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>The <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 protein is pivotal for <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation and heterochromatin formation, leading to decreased expressions of tumor suppressor genes and contributing to tumorigenesis. However, the factors that modulate <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 expression in colorectal cancer (<jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>) remain unclear. Here we showed that, compared with corresponding normal tissues, <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 was upregulated and micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u20109 (miR\u20109) was downregulated in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> tissues. The expression of <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 was inversely correlated with overall survival rates of patients with <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>. Overexpression of miR\u20109 in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cell lines significantly attenuated <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cell proliferation and promoted cell apoptosis. The expression of <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 was markedly reduced in pre\u2010miR\u20109 transfected <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cells. Using luciferase reporter assay, we confirmed that miR\u20109 was a direct upstream regulator of <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1. Finally, analysis of miR\u20109 and <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 levels in human <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> tissues revealed that expression of miR\u20109 was inversely correlated with <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1 expression. Collectively, our results offer <jats:italic>in\u00a0vitro<\/jats:italic> validation of the concept that miR\u20109 could repress the expression of <jats:styled-content style=\"fixed-case\">UHRF<\/jats:styled-content>1, and function as a tumor\u2010suppressive micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>. It may serve as a prognostic and therapeutic marker for <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/cas.12689","type":"journal-article","created":{"date-parts":[[2015,5,2]],"date-time":"2015-05-02T06:18:23Z","timestamp":1430547503000},"page":"833-839","source":"Crossref","is-referenced-by-count":57,"title":["Regulation of <scp>UHRF<\/scp>1 by micro<scp>RNA<\/scp>\u20109 modulates colorectal cancer cell proliferation and apoptosis"],"prefix":"10.1111","volume":"106","author":[{"given":"Mingchen","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Jiangsu Cancer Hospital &amp; Nanjing Medical University Cancer Hospital Nanjing China"},{"name":"Department of Pharmacology Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Nanjing First Hospital Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyuan","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Jiangsu Cancer Hospital &amp; Nanjing Medical University Cancer Hospital Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Gui","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Jiangsu Cancer Hospital &amp; Nanjing Medical University Cancer Hospital Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Jiangsu Cancer Hospital &amp; Nanjing Medical University Cancer Hospital Nanjing 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