{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:44:31Z","timestamp":1771476271800,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>Differentially regulated micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) are associated with hepatic fibrosis; however, their potential usefulness for blocking hepatic fibrosis has not been exploited fully. We examined the expression of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in the liver of a transgenic mouse model in which platelet\u2010derived growth factor C (<jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010C) is overexpressed (<jats:italic>Pdgf\u2010c Tg<\/jats:italic>), resulting in hepatic fibrosis and steatosis and the eventual development of hepatocellular carcinoma (<jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>). Robust induction of miR\u2010214 correlated with fibrogenesis in the liver of <jats:italic>Pdgf\u2010c Tg<\/jats:italic> mice, atherogenic high\u2010fat diet\u2010induced <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> mice, and patients with chronic hepatitis B or C. <jats:italic>Pdgf\u2010c Tg<\/jats:italic> mice were injected with locked nucleic acid (<jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>)\u2010antimiR\u2010214 via the tail vein using Invivofectamine 2.0 and the degree of hepatic fibrosis and tumor incidence were\u00a0evaluated. <jats:italic>Pdgf\u2010c Tg<\/jats:italic> mice treated with <jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>\u2010antimiR\u2010214 showed a marked reduction in fibrosis and tumor incidence compared with saline or <jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>\u2010miR\u2010control\u2010injected control mice. <jats:italic>In\u00a0vitro<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> LNA<\/jats:styled-content>\u2010antimiR\u2010214 significantly ameliorated <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21\u2010induced pro\u2010fibrotic gene expression in Lx\u20102 cells. MiR\u2010214 targets a negative regulator of <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> signaling, Mig\u20106. Mimic\u2010miR\u2010214 decreased the expression of Mig\u20106 and increased the levels of <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\u2010mediated p\u2010<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> (Y1173 and Y845) and p\u2010Met (Tyr1234\/1235) in Huh\u20107 cells. Conversely, <jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>\u2010antimiR\u2010214 repressed the expression of these genes. In conclusion, miR\u2010214 appears to participate in the development of hepatic fibrosis by modulating the <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 signaling pathways. <jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>\u2010antimiR\u2010214 is a potential therapy for the prevention of hepatic fibrosis.<\/jats:p>","DOI":"10.1111\/cas.12730","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T10:21:22Z","timestamp":1435573282000},"page":"1143-1152","source":"Crossref","is-referenced-by-count":43,"title":["Inhibition of micro<scp>RNA<\/scp>\u2010214 ameliorates hepatic fibrosis and tumor incidence in platelet\u2010derived growth factor C transgenic mice"],"prefix":"10.1111","volume":"106","author":[{"given":"Hikari","family":"Okada","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Kanazawa 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