{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:32:42Z","timestamp":1767846762997,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,9,19]],"date-time":"2015-09-19T00:00:00Z","timestamp":1442620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology, Japan","doi-asserted-by":"crossref","award":["23659432"],"award-info":[{"award-number":["23659432"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Okayama University Center of Excellence"},{"name":"Strategic Research Foundation Grant-aided Project for Private Universities"},{"name":"Grant-in-Aid from Novartis Pharma K.K, Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>Malignant mesothelioma is an aggressive and therapy\u2010resistant neoplasm arising from mesothelial cells. Evidence suggests that the major pathology associated with asbestos\u2010induced mesothelioma is local iron overload. In the present study, we induced iron\u2010induced mesothelioma in rats based on previous reports. Ten Wistar rats were given ferric saccharate and nitrilotriacetate i.p. for 5 days a week. Five of the ten rats exhibited widespread mesotheliomas in the peritoneum and tunica vaginalis. The tumor cells showed positive immunostaining for calretinin, wilms tumor\u20101, podoplanin and the oxidative <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> marker 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine. In three of the five rats with mesothelioma, array\u2010based comparative genomic hybridization analysis identified a common chromosomal deletion mapped to the chromosomal 4q31 locus, which encompasses the <jats:italic><jats:styled-content style=\"fixed-case\">TBXAS<\/jats:styled-content>1<\/jats:italic> gene. Downregulation of the <jats:italic><jats:styled-content style=\"fixed-case\">TBXAS<\/jats:styled-content>1<\/jats:italic> gene was confirmed using quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. <jats:italic><jats:styled-content style=\"fixed-case\">TBXAS<\/jats:styled-content>1<\/jats:italic> gene expression was also reduced in three of four human malignant pleural mesothelioma cell lines compared with normal bronchial epithelial cells. Immunohistochemistry revealed that <jats:italic><jats:styled-content style=\"fixed-case\">TBXAS<\/jats:styled-content>1<\/jats:italic> expression was weakly positive and positive in five and three out of eight human malignant mesothelioma samples, respectively. In conclusion, <jats:italic><jats:styled-content style=\"fixed-case\">TBXAS<\/jats:styled-content>1<\/jats:italic> gene expression was downregulated in rats with iron\u2010induced mesothelioma. The relationship between iron overload and <jats:italic><jats:styled-content style=\"fixed-case\">TBXAS<\/jats:styled-content>1<\/jats:italic> downregulation should be pursued further.<\/jats:p>","DOI":"10.1111\/cas.12752","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T16:16:52Z","timestamp":1437754612000},"page":"1296-1302","source":"Crossref","is-referenced-by-count":15,"title":["Downregulation of <i><scp>TBXAS<\/scp>1<\/i> in an iron\u2010induced malignant mesothelioma model"],"prefix":"10.1111","volume":"106","author":[{"given":"Daisuke","family":"Minami","sequence":"first","affiliation":[{"name":"Department of Hematology, Oncology and Respiratory Medicine Okayama University Hospital Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nagio","family":"Takigawa","sequence":"additional","affiliation":[{"name":"Department of General Internal Medicine Kawasaki Medical School Okayama 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