{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:58:53Z","timestamp":1792191533697,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,15]],"date-time":"2015-10-15T00:00:00Z","timestamp":1444867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005610","name":"Fukuoka University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002981","name":"Milj\u00f8ministeriet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002981","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Differentiating malignant pleural mesothelioma (MPM) cells morphologically from reactive mesothelial hyperplasia cells is problematic. Homozygous deletion (HD) of <jats:italic>p16<\/jats:italic> (<jats:italic>CDKN2A<\/jats:italic>), detected by FISH, is a good marker of malignancy and is useful to differentiate between these cells. However, the correlation between the <jats:italic>p16<\/jats:italic> status of effusion smears and that of the underlying MPM tissues has not been investigated. We used <jats:italic>p16<\/jats:italic>\u2010specific FISH to investigate 20 cases of MPM from which both effusion cytologic smears and histologic specimens were available. In five cases, histologic specimens included both an invasive component and surface mesothelial proliferation. In 14 cases (70%), MPM cells in both tissue sections and effusion smears were <jats:italic>p16<\/jats:italic> HD\u2010positive. Conversely, MPM cells in the remaining six tumors (30%) were <jats:italic>p16<\/jats:italic> HD\u2010negative in both tissue sections and effusion smears. For all five MPM cases with surface mesothelial proliferations and invasive components, the effusion smears, surface mesothelial proliferations, and invasive MPM components all displayed <jats:italic>p16<\/jats:italic> deletion. Moreover, the extent to which <jats:italic>p16<\/jats:italic> was deleted in smears highly correlated with the extent of <jats:italic>p16<\/jats:italic> deletion in tissues. The <jats:italic>p16<\/jats:italic> deletion percentages were also similar among smears, tissue surface proliferations, and invasive components. In cases with clinical and radiologic evidence of a diffuse pleural tumor, detection of <jats:italic>p16<\/jats:italic> deletion in cytologic smear samples may permit MPM diagnosis without additional tissue examination. However, the absence of <jats:italic>p16<\/jats:italic> deletion in cytologic smear samples does not preclude MPM.<\/jats:p>","DOI":"10.1111\/cas.12769","type":"journal-article","created":{"date-parts":[[2015,8,20]],"date-time":"2015-08-20T14:57:15Z","timestamp":1440082635000},"page":"1635-1641","source":"Crossref","is-referenced-by-count":39,"title":["Deletion status of <i>p16<\/i> in effusion smear preparation correlates with that of underlying malignant pleural mesothelioma tissue"],"prefix":"10.1111","volume":"106","author":[{"given":"Tomoyuki","family":"Hida","sequence":"first","affiliation":[{"name":"Department of Pathology Fukuoka University School of Medicine and Hospital Fukuoka Japan"},{"name":"Department of Anatomic Pathology Graduate School of Medical Sciences Kyushu University Fukuoka Japan"},{"name":"Department of Clinical Radiology Graduate School of Medical Sciences Kyushu University Fukuoka 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