{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:33:10Z","timestamp":1773405190497,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T00:00:00Z","timestamp":1444953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Cancer Center Research and Development Fund","award":["23-A-7"],"award-info":[{"award-number":["23-A-7"]}]},{"name":"Ministry of Health, Labor, and Welfare of Japan"},{"DOI":"10.13039\/501100013489","name":"Yakult Bio-science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100013489","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["15ck0106098h0002"],"award-info":[{"award-number":["15ck0106098h0002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Obesity is a risk factor for colorectal cancer. The accumulation of abdominal fat tissue causes abundant reactive oxygen species production through the activation of <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase due to excessive insulin stimulation. The enzyme <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase catalyzes the production of reactive oxygen species and evokes the initiation and progression of tumorigenesis. Apocynin is an <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase inhibitor that blocks the formation of the <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase complex (active form). In this study, we investigated the effects of apocynin on the development of azoxymethane\u2010induced colonic aberrant crypt foci in obese <jats:styled-content style=\"fixed-case\">KK<\/jats:styled-content>\u2010<jats:italic>A<\/jats:italic><jats:sup>y<\/jats:sup> mice and on the development of intestinal polyps in <jats:italic>Apc<\/jats:italic> mutant Min mice. Six\u2010week\u2010old <jats:styled-content style=\"fixed-case\">KK<\/jats:styled-content>\u2010<jats:italic>A<\/jats:italic><jats:sup>y<\/jats:sup> mice were injected with azoxymethane (200 \u03bcg\/mouse once per week for 3 weeks) and given 250 mg\/L apocynin or 500 mg\/L apocynin in their drinking water for 7 weeks. Six\u2010week\u2010old Min mice were also treated with 500 mg\/L apocynin for 6 weeks. Treatment with apocynin reduced the number of colorectal aberrant crypt foci in <jats:styled-content style=\"fixed-case\">KK<\/jats:styled-content>\u2010<jats:italic>A<\/jats:italic><jats:sup><jats:italic>y<\/jats:italic><\/jats:sup> mice by 21% and the number of intestinal polyps in Min mice by 40% compared with untreated mice. Both groups of mice tended to show improved oxidation of serum low\u2010density lipoprotein and 8\u2010oxo\u20102\u2032\u2010deoxyguanosine adducts in their adipose tissues. In addition, the inducible nitric oxide synthase <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels in polyp tissues decreased. Moreover, apocynin was shown to suppress nuclear factor\u2010\u03baB transcriptional activity <jats:italic>in vitro<\/jats:italic>. These results suggest that apocynin and other <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase inhibitors may be effective colorectal cancer chemopreventive agents.<\/jats:p>","DOI":"10.1111\/cas.12801","type":"journal-article","created":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T07:49:56Z","timestamp":1440661796000},"page":"1499-1505","source":"Crossref","is-referenced-by-count":20,"title":["Suppressive effects of the <scp>NADPH<\/scp> oxidase inhibitor apocynin on intestinal tumorigenesis in obese <scp>KK<\/scp>\u2010<i>A<\/i><sup><i>y<\/i><\/sup> and <i>Apc<\/i> mutant Min mice"],"prefix":"10.1111","volume":"106","author":[{"given":"Masami","family":"Komiya","sequence":"first","affiliation":[{"name":"Epidemiology and Prevention Division Research Center for Cancer Prevention and Screening National Cancer Center Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gen","family":"Fujii","sequence":"additional","affiliation":[{"name":"Division of Carcinogenesis and Cancer Prevention National Cancer Center Research Institute Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Epidemiology and Prevention Division Research Center for Cancer Prevention and Screening National Cancer Center Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mami","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Central Animal Division National Cancer Center Research Institute Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rikako","family":"Ishigamori","sequence":"additional","affiliation":[{"name":"Epidemiology and Prevention Division Research Center for Cancer Prevention and Screening National Cancer Center Tokyo 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