{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:08:18Z","timestamp":1790215698288,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T00:00:00Z","timestamp":1444953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["A14962"],"award-info":[{"award-number":["A14962"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>This study was designed to identify significant differences in gene expression profiles of human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>)\u2010positive and <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010negative oropharyngeal squamous cell carcinomas (<jats:styled-content style=\"fixed-case\">OPSCC<\/jats:styled-content>) and to better understand the functional and biological effects of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection in the premalignant pathway. Twenty\u2010four consecutive patients with locally advanced primary <jats:styled-content style=\"fixed-case\">OPSCC<\/jats:styled-content> were included in a prospective clinical trial. Fresh tissue samples (tumor <jats:italic>vs<\/jats:italic>. matched normal epithelium) were subjected to whole transcriptome analysis and the results validated on the same cohort with <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2013quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. In a separate retrospective cohort of 27 <jats:styled-content style=\"fixed-case\">OPSCC<\/jats:styled-content> patients, laser capture microdissection of formalin\u2010fixed, paraffin\u2010embedded tissue allowed <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> extraction from adjacent regions of normal epithelium, carcinoma <jats:italic>in situ<\/jats:italic> (premalignant) and invasive <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content> tissue. The majority of patients showed evidence of high\u2010risk <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16 positivity (80.4%). Predictable fold changes of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010associated disease included multiple transcripts within the p53 oncogenic pathway (e.g. <jats:italic><jats:styled-content style=\"fixed-case\">CDKN<\/jats:styled-content>2A<\/jats:italic>\/<jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic>). Other candidate transcripts found to have altered levels of expression in this study have not previously been established (<jats:italic><jats:styled-content style=\"fixed-case\">SFRP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">CRCT<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">DLG<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">SYCP<\/jats:styled-content>2<\/jats:italic>, and <jats:italic><jats:styled-content style=\"fixed-case\">CRNN<\/jats:styled-content><\/jats:italic>). Of these, <jats:italic><jats:styled-content style=\"fixed-case\">SYCP<\/jats:styled-content>2<\/jats:italic> showed the most consistent fold change from baseline in premalignant tissue; aberrant expression of this protein may contribute to genetic instability during <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010associated cancer development. If further corroborated, this data may contribute to the development of a non\u2010invasive screening tool. This study is registered with the <jats:styled-content style=\"fixed-case\">UK<\/jats:styled-content> Clinical Research Network (ref.: 11945).<\/jats:p>","DOI":"10.1111\/cas.12809","type":"journal-article","created":{"date-parts":[[2015,9,3]],"date-time":"2015-09-03T15:39:02Z","timestamp":1441294742000},"page":"1568-1575","source":"Crossref","is-referenced-by-count":54,"title":["Deregulation of <scp>SYCP<\/scp>2 predicts early stage human papillomavirus\u2010positive oropharyngeal carcinoma: A prospective whole transcriptome analysis"],"prefix":"10.1111","volume":"106","author":[{"given":"Liam","family":"Masterson","sequence":"first","affiliation":[{"name":"Department of Pathology University of Cambridge Cambridge UK"},{"name":"Department of Otorhinolaryngology Cambridge University Hospitals National Health Service Foundation Trust Cambridge 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