{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:33:27Z","timestamp":1787312007484,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T00:00:00Z","timestamp":1472083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["24591388"],"award-info":[{"award-number":["24591388"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["15K09473"],"award-info":[{"award-number":["15K09473"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2016,9]]},"abstract":"\n Genetic alterations in myelodysplastic syndromes (\n MDS<\/jats:styled-content>\n ) are critical for pathogenesis. We previously showed that peripheral blood cell\u2010free\n DNA<\/jats:styled-content>\n (\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n ) may be more sensitive for genetic\/epigenetic analyses than whole bone marrow (\n BM<\/jats:styled-content>\n ) cells and mononuclear cells in peripheral blood (\n PB<\/jats:styled-content>\n ). Here we analyzed the detailed features of\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n and its utility in genetic analyses in\n MDS<\/jats:styled-content>\n . The plasma\u2010\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n concentration in\n MDS<\/jats:styled-content>\n and related diseases (\n N<\/jats:italic>\n = 33) was significantly higher than that in healthy donors (\n N<\/jats:italic>\n = 14;\n P =<\/jats:italic>\n 0.041) and in International Prognostic Scoring System higher\u2010risk groups than that in lower\u2010risk groups (\n P =<\/jats:italic>\n 0.034). The concentration of plasma\u2010\/serum\u2010\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n was significantly correlated with the serum lactate dehydrogenase level (both\n P<\/jats:italic>\n < 0.0001) and the blast cell count in\n PB<\/jats:styled-content>\n (\n P =<\/jats:italic>\n 0.034 and 0.025, respectively). One nanogram of\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n was sufficient for one assay of Sanger sequencing using optimized primer sets to amplify approximately 160\u2010bp\n PCR<\/jats:styled-content>\n products.\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n (approximately 50 ng) can also be utilized for targeted sequencing. Almost all mutations detected in\n BM<\/jats:styled-content>\n \u2010\n DNA<\/jats:styled-content>\n were also detected using corresponding\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n . Analyses using serially harvested\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n from an\n RAEB<\/jats:styled-content>\n \u20102 patient showed that the somatic mutations and a single nucleotide polymorphism that were detected before allogeneic transplantation were undetectable after transplantation, indicating that\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n likely comes from\n MDS<\/jats:styled-content>\n clones that reflect the disease status.\n PB<\/jats:styled-content>\n cf\n DNA<\/jats:styled-content>\n may be a safer and easier alternative to obtain tumor\n DNA<\/jats:styled-content>\n in\n MDS<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/cas.12994","type":"journal-article","created":{"date-parts":[[2016,6,21]],"date-time":"2016-06-21T03:25:30Z","timestamp":1466479530000},"page":"1329-1337","source":"Crossref","is-referenced-by-count":21,"title":["Peripheral blood cell\u2010free\n DNA<\/scp>\n is an alternative tumor\n DNA<\/scp>\n source reflecting disease status in myelodysplastic syndromes"],"prefix":"10.1111","volume":"107","author":[{"given":"Yasuhiro","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Tomita","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumika","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"},{"name":"Department of Analytical Neurobiology Faculty of Pharmacy Meijo University Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chisako","family":"Iriyama","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuho","family":"Shirahata\u2010Adachi","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyuki","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"},{"name":"Institute for Adavanced Research Nagoya University Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akimi","family":"Akashi","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Kaneda","sequence":"additional","affiliation":[{"name":"Department of Analytical Neurobiology Faculty of Pharmacy Meijo University Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Kiyoi","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya 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