{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:34:08Z","timestamp":1791423248682,"version":"4.3.3"},"reference-count":62,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2016,10]]},"abstract":"The breast and ovarian cancer predisposition protein BRCA<\/jats:styled-content>1 forms three mutually exclusive complexes with Fanconi anemia group J protein (FANCJ<\/jats:styled-content>, also called BACH<\/jats:styled-content>1 or BRIP<\/jats:styled-content>1), CtIP<\/jats:styled-content>, and Abraxas\/RAP<\/jats:styled-content>80 through its BRCA<\/jats:styled-content>1 C terminus (BRCT<\/jats:styled-content>) domains, while its RING<\/jats:styled-content> domain binds to BRCA<\/jats:styled-content>1\u2010associated RING<\/jats:styled-content> domain 1 (BARD<\/jats:styled-content>1). We recently found that the interaction between heterochromatin protein 1 (HP1) and BARD<\/jats:styled-content>1 is required for the accumulation of BRCA<\/jats:styled-content>1 and CtIP<\/jats:styled-content> at sites of DNA<\/jats:styled-content> double\u2010strand breaks. Here, we investigated the importance of HP<\/jats:styled-content>1 and BARD<\/jats:styled-content>1\u2013HP<\/jats:styled-content>1 interaction in the localization of FANCJ<\/jats:styled-content> together with the other BRCA<\/jats:styled-content>1\u2013BRCT<\/jats:styled-content> binding proteins to clarify the separate role of the HP<\/jats:styled-content>1\u2010mediated pathway from the RNF8\/RNF<\/jats:styled-content>168\u2010induced ubiquitin\u2010mediated pathway for BRCA<\/jats:styled-content>1 function. FANCJ<\/jats:styled-content> interacts with HP<\/jats:styled-content>1\u03b3 in a BARD<\/jats:styled-content>1\u2010dependent manner, and this interaction was enhanced by ionizing radiation or irinotecan hydrochloride treatment. Simultaneous depletion of all three HP<\/jats:styled-content>1 isoforms with shRNA<\/jats:styled-content>s disrupts the accumulation of FANCJ<\/jats:styled-content> and CtIP<\/jats:styled-content>, but not RAP<\/jats:styled-content>80, at double\u2010strand break sites. Replacement of endogenous BARD<\/jats:styled-content>1 with a mutant BARD<\/jats:styled-content>1 that is incapable of binding to HP<\/jats:styled-content>1 also disrupts the accumulation of FANCJ<\/jats:styled-content> and CtIP<\/jats:styled-content>, but not RAP<\/jats:styled-content>80. In contrast, RNF<\/jats:styled-content>168 depletion disrupts the accumulation of only RAP<\/jats:styled-content>80, but not FANCJ<\/jats:styled-content> or CtIP<\/jats:styled-content>. Consequently, the accumulation of conjugated ubiquitin was only inhibited by RNF<\/jats:styled-content>168 depletion, whereas the accumulation of RAD<\/jats:styled-content>51 and sister chromatid exchange were only inhibited by HP<\/jats:styled-content>1 depletion or disruption of the BARD<\/jats:styled-content>1\u2013HP<\/jats:styled-content>1 interaction. Taken together, the results suggest that the BRCA<\/jats:styled-content>1\u2013FANCJ<\/jats:styled-content> and BRCA<\/jats:styled-content>1\u2013CtIP<\/jats:styled-content> complexes are not downstream of the RNF<\/jats:styled-content>8\/RNF<\/jats:styled-content>168\/ubiquitin pathway, but are instead regulated by the HP<\/jats:styled-content>1 pathway that precedes homologous recombination DNA<\/jats:styled-content> repair.<\/jats:p>","DOI":"10.1111\/cas.13008","type":"journal-article","created":{"date-parts":[[2016,7,11]],"date-time":"2016-07-11T12:54:18Z","timestamp":1468241658000},"page":"1406-1415","source":"Crossref","is-referenced-by-count":16,"title":["HP1 regulates the localization of FANCJ at sites of DNA<\/scp> double\u2010strand breaks"],"prefix":"10.1111","volume":"107","author":[{"given":"Wenwen","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Togashi","sequence":"additional","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Johmura","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Graduate School of Medical Sciences Nagoya City University Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Division of Breast and Endocrine Surgery Department of Surgery Hyogo College of Medicine Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachihiko","family":"Nobuoka","sequence":"additional","affiliation":[{"name":"Laboratory Medicine St. Marianna University Graduate School of Medicine Kawasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Graduate School of Medical Sciences Nagoya City University Nagoya Japan"},{"name":"Division of Cancer Cell Biology The Institute of Medical Science The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiko","family":"Ohta","sequence":"additional","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki 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