{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:32:39Z","timestamp":1791613959079,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31100641"],"award-info":[{"award-number":["31100641"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270946"],"award-info":[{"award-number":["31270946"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>There is increasing evidence that cytoskeleton remodeling is involved in cancer progression. Wiskott\u2010Aldrich syndrome protein (<jats:styled-content style=\"fixed-case\">WASP<\/jats:styled-content>) family represents a key regulator of actin cytoskeleton remodeling. However, the underlying mechanism of the <jats:styled-content style=\"fixed-case\">WASP<\/jats:styled-content> family in cancer progression remains elusive. Here, we studied the role of <jats:styled-content style=\"fixed-case\">WASP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SCAR<\/jats:styled-content> Homolog (<jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>), a recently identified <jats:styled-content style=\"fixed-case\">WASP<\/jats:styled-content> family member, in human esophageal squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">ESCC<\/jats:styled-content>). Using three human <jats:styled-content style=\"fixed-case\">ESCC<\/jats:styled-content> cell lines, we found that <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content> expression was significantly elevated in cancer stem\u2010like cells enriched by sphere formation assay. <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content> knockdown decreased the sphere\u2010forming capacity of esophageal cancer cells whereas <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content> over\u2010expression exhibited the opposite effect. Mechanistically, we identified interleukin\u20108 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108) as a key downstream target of <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 knockdown completely attenuated tumor sphere formation induced by <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content> overexpression. <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content> knockdown also delayed the growth of human <jats:styled-content style=\"fixed-case\">ESCC<\/jats:styled-content> xenografts in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c nude mice. Importantly, high <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content> levels were associated with poor clinical prognosis in a total of 145 human <jats:styled-content style=\"fixed-case\">ESCC<\/jats:styled-content> tissues. Collectively, our results suggest an essential role of the <jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 pathway in human <jats:styled-content style=\"fixed-case\">ESCC<\/jats:styled-content> by maintaining the stemness of cancer cells. Hence, targeting this pathway might represent a promising strategy to control human esophageal carcinoma.<\/jats:p>","DOI":"10.1111\/cas.13400","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T10:32:46Z","timestamp":1505471566000},"page":"2358-2365","source":"Crossref","is-referenced-by-count":18,"title":["<scp>WASH<\/scp> overexpression enhances cancer stem cell properties and correlates with poor prognosis of esophageal carcinoma"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-4067","authenticated-orcid":false,"given":"Lan","family":"Huang","sequence":"first","affiliation":[{"name":"Biotherapy Center The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyao","family":"Lian","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University Zhengzhou 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