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Although many systems detect <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>, a gold standard has not yet been established. We analyzed <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> in uterine cervical cancer patients using an advanced version of conditionally replicative adenovirus targeting telomerase\u2010positive cells, which was enabled to infect coxsackievirus\u2010adenovirus receptor\u2010negative cells and to reduce false\u2010positive signals in myeloid cells. Blood samples from cervical cancer patients were hemolyzed and infected with the virus and then labeled with fluorescent anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45 and anti\u2010pan cytokeratin antibodies. <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> (+)\/<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45 (\u2212) cells were isolated and subjected to whole\u2010genome amplification followed by polymerase chain reaction analysis of human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> were detected in 6 of 23 patients with cervical cancers (26.0%). Expression of <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> did not correlate with the stage of cancer or other clinicopathological factors. In 5 of the 6 <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010positive cases, the same subtype of <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content> as that of the corresponding primary lesion was detected, indicating that the <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> originated from <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010infected cancer cells. These <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> were all negative for cytokeratins. The <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> detected by our system were genetically confirmed. <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> derived from uterine cervical cancers had lost epithelial characteristics, indicating that epithelial marker\u2010dependent systems do not have the capacity to detect these cells in cervical cancer patients.<\/jats:p>","DOI":"10.1111\/cas.13449","type":"journal-article","created":{"date-parts":[[2017,11,19]],"date-time":"2017-11-19T13:12:41Z","timestamp":1511097161000},"page":"231-240","source":"Crossref","is-referenced-by-count":26,"title":["Detection of circulating tumor cells in cervical cancer using a conditionally replicative adenovirus targeting telomerase\u2010positive cells"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3707-8691","authenticated-orcid":false,"given":"Masahiro","family":"Takakura","sequence":"first","affiliation":[{"name":"Department 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