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Somatic RHOA<\/jats:styled-content><\/jats:italic> mutations, most frequently found at the hotspot site c.50G\u00a0>\u00a0T, p.Gly17Val (G17V RHOA<\/jats:styled-content><\/jats:italic> mutation) are a genetic hallmark of AITL<\/jats:styled-content>. Detection of the G17V RHOA<\/jats:styled-content><\/jats:italic> mutations assists prompt and appropriate diagnosis of AITL<\/jats:styled-content>. However, an optimal detection method for the G17V RHOA<\/jats:styled-content><\/jats:italic> mutation remains to be elucidated. We compared the sensitivity and concordance of next\u2010generation sequencing (NGS<\/jats:styled-content>), droplet digital PCR<\/jats:styled-content> (ddPCR<\/jats:styled-content>) and peptide nucleic acid\u2010locked nucleic acid (PNA<\/jats:styled-content>\u2010LNA<\/jats:styled-content>) clamp method for detecting the G17V RHOA<\/jats:styled-content><\/jats:italic> mutation. G17V RHOA<\/jats:styled-content><\/jats:italic> mutations were identified in 27 of 67 (40.3%) PTCL<\/jats:styled-content> samples using NGS<\/jats:styled-content>. ddPCR<\/jats:styled-content> and PNA<\/jats:styled-content>\u2010LNA<\/jats:styled-content> clamp method both detected G17V mutations in 4 samples in addition to those detected with NGS<\/jats:styled-content> (31 of 67, 46.3%). Additionally, variant allele frequencies with ddPCR<\/jats:styled-content> and those with NGS<\/jats:styled-content> showed high concordance (P\u00a0<\/jats:italic><\u00a0.001). Three other RHOA<\/jats:styled-content><\/jats:italic> mutations involving the p.Gly17 position (c.[49G\u00a0>\u00a0T;50G\u00a0>\u00a0T], p.Gly17Leu in PTCL<\/jats:styled-content>198; c.[50G\u00a0>\u00a0T;51A\u00a0>\u00a0C], p.Gly17Val in PTCL<\/jats:styled-content>216; and c.50G\u00a0>\u00a0A, p.Gly17Glu in PTCL<\/jats:styled-content>223) were detected using NGS<\/jats:styled-content>. These sequence changes could not appropriately be detected using the ddPCR<\/jats:styled-content> assay and the PNA<\/jats:styled-content>\u2010LNA<\/jats:styled-content> clamp method although both indicated that the samples might have mutations. In total, 34 out of 67 PTCL<\/jats:styled-content> samples (50.7%) had RHOA<\/jats:styled-content><\/jats:italic> mutations at the p.Gly17 position. In conclusion, our results suggested that a combination of ddPCR<\/jats:styled-content>\/PNA<\/jats:styled-content>\u2010LNA<\/jats:styled-content> clamp methods and NGS<\/jats:styled-content> are best method to assist the diagnosis of AITL<\/jats:styled-content> by detecting RHOA<\/jats:styled-content><\/jats:italic> mutations at the p.Gly17 position.<\/jats:p>","DOI":"10.1111\/cas.13557","type":"journal-article","created":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T14:52:43Z","timestamp":1519915963000},"page":"1682-1689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Droplet digital polymerase chain reaction assay and peptide nucleic acid\u2010locked nucleic acid clamp method for RHOA<\/scp><\/i> mutation detection in angioimmunoblastic T\u2010cell lymphoma"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1516-9568","authenticated-orcid":false,"given":"Sharna","family":"Tanzima Nuhat","sequence":"first","affiliation":[{"name":"Department of Hematology Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamiko","family":"Sakata\u2010Yanagimoto","sequence":"additional","affiliation":[{"name":"Department of Hematology Faculty of Medicine University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Komori","sequence":"additional","affiliation":[{"name":"Department of Hematology Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0810-7887","authenticated-orcid":false,"given":"Keiichiro","family":"Hattori","sequence":"additional","affiliation":[{"name":"Department of Hematology Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhito","family":"Suehara","sequence":"additional","affiliation":[{"name":"Department of Hematology Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2729-5780","authenticated-orcid":false,"given":"Kota","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"Department of Hematology Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Fujisawa","sequence":"additional","affiliation":[{"name":"Department of Hematology Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2518-0776","authenticated-orcid":false,"given":"Manabu","family":"Kusakabe","sequence":"additional","affiliation":[{"name":"Department of Hematology Faculty of Medicine University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8669-9865","authenticated-orcid":false,"given":"Kosei","family":"Matsue","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology Department of Internal Medicine Kameda Medical Center Kamogawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotake","family":"Wakamatsu","sequence":"additional","affiliation":[{"name":"LSI Medience Co. 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