{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:01:13Z","timestamp":1784674873871,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T00:00:00Z","timestamp":1524096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009734","name":"Olympus","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009734","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009483","name":"Institute of Global Innovation Research, Tokyo University of Agriculture and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009483","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>Fluorescence tumor imaging using exogenous fluorescent tumor\u2010targeting agents has potential to improve early tumor detection. The fluorescent contrast agent indocyanine green (<jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content>) is used in medical diagnostics. The aim of the present study is to investigate the tumor imaging capability and the imaging mechanism of i.v. administered <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content> in a mouse model of colitis\u2010associated colon cancer. To do this, an azoxymethane\/dextran sodium sulfate\u2010induced colon cancer mouse model was used. Ex vivo imaging experiments were carried out 1\u00a0hour after i.v. injection of <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content> fluorescence was observed in the colon tumor tissues, with sufficient tumor to normal tissue ratio, correlating with tumor malignancy. In the tumor tissues, <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content> fluorescence was localized in the vascular interstitial tissue. Immunofluorescence microscopy revealed that tumor cells formed tight junctions normally, suggesting an inability of tumor cellular uptake of <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content>. In contrast, tumor tissues increased the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>31\u2010immunoreactive endothelial cell area, and accumulated stromal cells immunoreactive for <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 and tumor cell population immunoreactive for inducible nitric oxide synthase. In vivo vascular permeability assay revealed that prostaglandin E<jats:sub>2<\/jats:sub> promoted the endothelial cell permeability of <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content>. In conclusion, our data indicated that fluorescence contrast\u2010enhanced imaging following i.v. administered <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content> can be applied to the detection of colon tumors in a mouse colitis\u2010associated colon cancer model. The tumor tissue preference of <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content> in the present model can be attributed to the enhanced vascular leakage of <jats:styled-content style=\"fixed-case\">ICG<\/jats:styled-content> involving inflammatory mediators, such as <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 and inducible nitric oxide synthase, in conjunction with increased tumor vascularity.<\/jats:p>","DOI":"10.1111\/cas.13564","type":"journal-article","created":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T05:36:41Z","timestamp":1520573801000},"page":"1638-1647","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Fluorescence tumor imaging by i.v. administered indocyanine green in a mouse model of colitis\u2010associated colon cancer"],"prefix":"10.1111","volume":"109","author":[{"given":"Rei","family":"Nagahara","sequence":"first","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuhiko","family":"Onda","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"},{"name":"Evaluation Technology Department 1 R&amp;D Group Olympus Corporation Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Evaluation Technology Department 1 R&amp;D Group Olympus Corporation Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Kojima","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"},{"name":"Evaluation Technology Department 1 R&amp;D Group Olympus Corporation Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Inohana","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayumi","family":"Eguchi","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misato","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinya","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Evaluation Technology Department 1 R&amp;D Group Olympus Corporation Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshinori","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3417-9697","authenticated-orcid":false,"given":"Makoto","family":"Shibutani","sequence":"additional","affiliation":[{"name":"Division of Animal Life Science Laboratory of Veterinary Pathology Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan"},{"name":"Institute of Global Innovation Research Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-012-0352-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/den.12505"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4253\/wjge.v9.i8.359"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301795"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.125"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3566"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(16)30027-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06917"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr900263j"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2310\/7290.2009.00031"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.21272"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24291"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040570597"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S42006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2014.05.026"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129766"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2016.06.389"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000001618"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.24318"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00464-016-5007-6"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-013-3360-4"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2014.07.055"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.07.047"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30102"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.21744"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26113"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-1188"},{"key":"e_1_2_8_29_1","first-page":"483","article-title":"Dose\u2010dependent promoting effect of dextran sodium sulfate on mouse colon carcinogenesis initiated with azoxymethane","volume":"20","author":"Suzuki R","year":"2005","journal-title":"Histol Histopathol"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2007.00695.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/658786"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2016.12.018"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12815"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.88.53"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2008.01003.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2003.tb01386.x"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23923"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-824X-4-18"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.10.002"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcas.13564","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.13564","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cas.13564","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.13564","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T04:04:10Z","timestamp":1693713850000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cas.13564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,19]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/cas.13564"],"URL":"https:\/\/doi.org\/10.1111\/cas.13564","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,19]]},"assertion":[{"value":"2017-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}