{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T09:21:14Z","timestamp":1758705674422,"version":"3.37.3"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T00:00:00Z","timestamp":1529625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["\u201cH27\u2010kagaku\u2010shitei\u2010004,\u201d","\u201cH25\u2010 kagaku\u2010ippan\u2010004,\u201d","\u201cH28\u2010kagaku\u2010ippan\u2010004\u201d"],"award-info":[{"award-number":["\u201cH27\u2010kagaku\u2010shitei\u2010004,\u201d","\u201cH25\u2010 kagaku\u2010ippan\u2010004,\u201d","\u201cH28\u2010kagaku\u2010ippan\u2010004\u201d"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007215","name":"Verband der Chemischen Industrie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007215","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004819","name":"Cultural Affairs and Missions Sector, Ministry of Higher Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2018,7]]},"abstract":"Potassium octatitanate fibers (K2<\/jats:sub>O\u00b78TiO2<\/jats:sub>, POT<\/jats:styled-content> fibers) are widely used as an alternative to asbestos. We investigated the pulmonary and pleural toxicity of POT<\/jats:styled-content> fibers with reference to 2 non\u2010fibrous titanium dioxide nanoparticles (nTiO<\/jats:styled-content>2<\/jats:sub>), photoreactive anatase (a\u2010nTiO<\/jats:styled-content>2<\/jats:sub>) and inert rutile (r\u2010nTiO<\/jats:styled-content>2<\/jats:sub>). Ten\u2010week\u2010old male F344 rats were given 0.5 mL<\/jats:styled-content> of 250 \u03bcg\/mL<\/jats:styled-content> suspensions of POT<\/jats:styled-content> fibers, a\u2010nTiO<\/jats:styled-content>2<\/jats:sub>, or r\u2010nTiO<\/jats:styled-content>2<\/jats:sub>, 8 times (1 mg\/rat) over a 15\u2010day period by trans\u2010tracheal intrapulmonary spraying (TIPS<\/jats:styled-content>). Rats were killed at 6 hours and at 4 weeks after the last TIPS<\/jats:styled-content> dose. Alveolar macrophages were significantly increased in all treatment groups at 6 hours and at 4 weeks. At week 4, a\u2010nTiO<\/jats:styled-content>2<\/jats:sub> and r\u2010nTiO<\/jats:styled-content>2<\/jats:sub> were largely cleared from the lung whereas a major fraction of POT<\/jats:styled-content> fibers were not cleared. In the bronchoalveolar lavage, alkaline phosphatase activity was elevated in all treatment groups, and lactate dehydrogenase (LDH<\/jats:styled-content>) activity was elevated in the a\u2010nTiO<\/jats:styled-content>2<\/jats:sub> and POT<\/jats:styled-content> groups. In lung tissue, oxidative stress index and proliferating cell nuclear antigen (PCNA<\/jats:styled-content>) index were elevated in the a\u2010nTiO<\/jats:styled-content>2<\/jats:sub> and POT<\/jats:styled-content> groups, and there was a significant elevation in C\u2010C motif chemokine ligand 2 (CCL<\/jats:styled-content>2) mRNA<\/jats:styled-content> and protein in the POT<\/jats:styled-content> group. In pleural cavity lavage, total protein was elevated in all 3 treatment groups, and LDH<\/jats:styled-content> activity was elevated in the a\u2010nTiO<\/jats:styled-content>2<\/jats:sub> and POT<\/jats:styled-content> groups. Importantly, the PCNA<\/jats:styled-content> index of the visceral mesothelium was increased in the POT<\/jats:styled-content> group. Overall, POT<\/jats:styled-content> fibers had greater biopersistence, induced higher expression of CCL<\/jats:styled-content>2, and provoked a stronger tissue response than a\u2010nTiO<\/jats:styled-content>2<\/jats:sub> or r\u2010nTiO<\/jats:styled-content>2<\/jats:sub>.<\/jats:p>","DOI":"10.1111\/cas.13643","type":"journal-article","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T05:46:43Z","timestamp":1526622403000},"page":"2164-2177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Potassium octatitanate fibers induce persistent lung and pleural injury and are possibly carcinogenic in male Fischer 344 rats"],"prefix":"10.1111","volume":"109","author":[{"given":"Mohamed","family":"Abdelgied","sequence":"first","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"},{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences Nagoya Japan"},{"name":"Department of Forensic Medicine and Toxicology Faculty of Veterinary Medicine Beni Suef University Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M.","family":"El\u2010Gazzar","sequence":"additional","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"},{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences Nagoya Japan"},{"name":"Department of Forensic Medicine and Toxicology Faculty of Veterinary Medicine Alexandria University Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9017-3639","authenticated-orcid":false,"given":"David B.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William T.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takamasa","family":"Numano","sequence":"additional","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Iigou","sequence":"additional","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aya","family":"Naiki\u2010Ito","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotsugu","family":"Takase","sequence":"additional","affiliation":[{"name":"Core Laboratory Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khaled Abbas","family":"Abdou","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology Faculty of Veterinary Medicine Beni Suef University Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Hirose","sequence":"additional","affiliation":[{"name":"Division of Risk Assessment National Institute of Health Sciences Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhji","family":"Taquahashi","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Toxicology National Institute of Health Sciences Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Kanno","sequence":"additional","affiliation":[{"name":"Japan Industrial Safety and Health Association Japan Bioassay Research Center Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Tsuda","sequence":"additional","affiliation":[{"name":"Nanotoxicology Project Nagoya City University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1539\/joh.44.34"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.10.139"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2486\/indhealth.39.168"},{"key":"e_1_2_7_5_1","first-page":"143","volume-title":"Workshop on Asbestos: Definitions and Measurement Methods","author":"Stanton MF","year":"1978"},{"key":"e_1_2_7_6_1","first-page":"965","article-title":"Relation of particle dimension to carcinogenicity in amphibole asbestoses and other fibrous minerals","volume":"67","author":"Stanton MF","year":"1981","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1091581815587744"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12944"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/08958370490428391"},{"key":"e_1_2_7_10_1","first-page":"314","article-title":"Pulmonary response and transmigration of inorganic fibers by inhalation exposure","volume":"102","author":"Lee KP","year":"1981","journal-title":"Am J Pathol"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0013-9351(81)90143-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1539\/joh.46.382"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/08958370390248969"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-4878(94)00126-L"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2006.06.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2016.07.019"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.38.619"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2003.11.015"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2005.08.008"},{"key":"e_1_2_7_22_1","first-page":"563","article-title":"Increased oxidative stress in patients with hydatidiform mole","volume":"133","author":"Harma M","year":"2003","journal-title":"Swiss Med Wkly"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djq044"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14277"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-069X-13-59"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar300092y"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-9-47"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2013.07.014"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00139.2003"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-6-35"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2008.0027"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2006.12.012"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0609385"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2017.02.001"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.4071052"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-069X-7-4"},{"key":"e_1_2_7_37_1","unstructured":"NIOSH.Current Intelligence Bulletin 62. Asbestos Fibers and Other Elongate Mineral Particles: State of the Science and Road map for Research.2011.https:\/\/www.cdc.gov\/niosh\/docs\/2011-159\/pdfs\/2011-159.pdf. Accessed 24 Oct 2017. 2011."}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcas.13643","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.13643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T09:49:02Z","timestamp":1707299342000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cas.13643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,22]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/cas.13643"],"URL":"https:\/\/doi.org\/10.1111\/cas.13643","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"type":"print","value":"1347-9032"},{"type":"electronic","value":"1349-7006"}],"subject":[],"published":{"date-parts":[[2018,6,22]]},"assertion":[{"value":"2018-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}