{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:38:22Z","timestamp":1790041102437,"version":"4.0.1"},"reference-count":70,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T00:00:00Z","timestamp":1544745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004685","name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004685","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2019,1]]},"abstract":"\n T\u2010cell acute lymphoblastic leukemia (T\u2010\n ALL<\/jats:styled-content>\n ) has a poor prognosis derived from its genetic heterogeneity, which translates to a high chemoresistance. Recently, our workgroup designed thrombospondin\u20101\u2010derived\n CD<\/jats:styled-content>\n 47 agonist peptides and demonstrated their ability to induce cell death in chronic lymphocytic leukemia. Encouraged by these promising results, we evaluated cell death induced by\n PKHB<\/jats:styled-content>\n 1 (the first\u2010described serum\u2010stable\n CD<\/jats:styled-content>\n 47\u2010agonist peptide) on\n CEM<\/jats:styled-content>\n and\n MOLT<\/jats:styled-content>\n \u20104 human cell lines (T\u2010\n ALL<\/jats:styled-content>\n ) and on one T\u2010murine tumor lymphoblast cell\u2010line (L5178Y\u2010R), also assessing caspase and calcium dependency and mitochondrial membrane potential. Additionally, we evaluated selectivity for cancer cell lines by analyzing cell death and viability of human and murine non\u2010tumor cells after\n CD<\/jats:styled-content>\n 47 activation. In\u00a0vivo, we determined that\n PKHB<\/jats:styled-content>\n 1\u2010treatment in mice bearing the L5178Y\u2010R cell line increased leukocyte cell count in peripheral blood and lymphoid organs while recruiting leukocytes to the tumor site. To analyze whether\n CD<\/jats:styled-content>\n 47 activation induced immunogenic cell death (\n ICD<\/jats:styled-content>\n ), we evaluated damage\u2010associated molecular patterns (\n DAMP<\/jats:styled-content>\n ) exposure (calreticulin,\n CRT<\/jats:styled-content>\n ) and release (\n ATP<\/jats:styled-content>\n , heat shock proteins 70 and 90, high\u2010mobility group box 1,\n CRT<\/jats:styled-content>\n ). Furthermore, we gave prophylactic antitumor vaccination, determining immunological memory. Our data indicate that\n PKHB<\/jats:styled-content>\n 1 induces caspase\u2010independent and calcium\u2010dependent cell death in leukemic cells while sparing non\u2010tumor murine and human cells. Moreover, our results show that\n PKHB<\/jats:styled-content>\n 1 can induce\n ICD<\/jats:styled-content>\n in leukemic cells as it induces\n CRT<\/jats:styled-content>\n exposure and\n DAMP<\/jats:styled-content>\n release in\u00a0vitro, and prophylactic vaccinations inhibit tumor establishment in\u00a0vivo. Together, our results improve the knowledge of\n CD<\/jats:styled-content>\n 47 agonist peptides potential as therapeutic tools to treat leukemia.\n <\/jats:p>","DOI":"10.1111\/cas.13885","type":"journal-article","created":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T20:14:36Z","timestamp":1542744876000},"page":"256-268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["CD<\/scp>\n 47 agonist peptide\n PKHB<\/scp>\n 1 induces immunogenic cell death in T\u2010cell acute lymphoblastic leukemia cells"],"prefix":"10.1111","volume":"110","author":[{"given":"Ashanti Concepci\u00f3n","family":"Uscanga\u2010Palomeque","sequence":"first","affiliation":[{"name":"College of Biology Science Laboratory of Immunology and Virology Autonomus University of Nuevo Leon San Nicolas de los Garza Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenny 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