{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T02:44:23Z","timestamp":1761965063736,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K10697","16H01747"],"award-info":[{"award-number":["16K10697","16H01747"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007206","name":"Kaibara Morikazu Medical Science Promotion Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>Detection of rare tumor cells circulating in the blood (<jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>s) presents technical challenges. CellSearch, the only approved system for clinical use, fails to capture epithelial cell adhesion molecule\u2010negative <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>s such as malignant pleural mesothelioma (<jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>). We have developed a novel microfluidic device (<jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010chip) in which any Ab to capture <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>s is conjugated. The <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010chip was coated with an Ab against podoplanin that is abundantly expressed on <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">Circulating tumor cell<\/jats:styled-content>\u2010detection performance was evaluated in experimental models in which <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> cells were spiked in blood sampled from a healthy volunteer and in clinical samples drawn from <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> patients. The <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010chip showed superior <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010detection performance over CellSearch in experimental models (sensitivity, 63.3%\u201064.5% vs 0%\u20101.1%; <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.001) and in clinical samples (<jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010positivity, 68.8% vs 6.3%; <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.001). A receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) analysis showed that the <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> test provided a significant diagnostic performance in discrimination of unresectable disease from resectable disease (area under the <jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content> curve, 0.851; <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.003). The higher <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content> count (\u22652\u00a0cells\/mL) was significantly associated with a poor prognosis (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.030). The novel <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>\u2010chip enabled sensitive detection of <jats:styled-content style=\"fixed-case\">CTC<\/jats:styled-content>s, which provided significant diagnostic and prognostic information in <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/cas.13895","type":"journal-article","created":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T08:36:35Z","timestamp":1543566995000},"page":"726-733","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Detection of circulating tumor cells with a novel microfluidic system in malignant pleural mesothelioma"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7142-2743","authenticated-orcid":false,"given":"Kazue","family":"Yoneda","sequence":"first","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiji","family":"Kuwata","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Chikaishi","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Mori","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Kanayama","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Takenaka","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soichi","family":"Oka","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Hirai","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Imanishi","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinobu","family":"Ichiki","sequence":"additional","affiliation":[{"name":"Second Department of Surgery (Chest Surgery) University of Occupational and Environmental Health  Japan Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Ohnaga","sequence":"additional","affiliation":[{"name":"Central Research Laboratories Toyama Industrial Technology Center  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