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As TGF<\/jats:styled-content>\u2010\u03b2\/Smad signaling is considered to play a key role in epithelial\u2010mesenchymal transition (EMT<\/jats:styled-content>), we attempted to clarify the mechanism underlying LRG<\/jats:styled-content>\u2010related EMT<\/jats:styled-content> in relation to metastasis in PDAC<\/jats:styled-content>. We cultured LRG<\/jats:styled-content>\u2010overexpressing PDAC<\/jats:styled-content> cells (Panc1\/LRG<\/jats:styled-content>) and evaluated the morphology, EMT<\/jats:styled-content>\u2010related molecules and TGF<\/jats:styled-content>\u2010\u03b2\/Smad signaling pathway in these cells. We also assessed the LRG<\/jats:styled-content> levels in plasma and resected specimens from patients with PDAC<\/jats:styled-content>. Inflammatory cytokines induced LRG<\/jats:styled-content> production in PDAC<\/jats:styled-content> cells. A spindle\u2010like shape was visualized more frequently than other shapes in Panc1\/LRG<\/jats:styled-content> with TGF<\/jats:styled-content>\u2010\u03b21 exposure. The expression of E\u2010cadherin in Panc1\/LRG<\/jats:styled-content> was decreased with TGF<\/jats:styled-content>\u2010\u03b21 exposure. Invasion increased with TGF<\/jats:styled-content>\u2010\u03b21 stimulation of Panc1\/LRG<\/jats:styled-content>. The phosphorylation of smad2 in Panc1\/LRG<\/jats:styled-content> was increased in comparison with parental Panc1 under TGF<\/jats:styled-content>\u2010\u03b21 stimulation. In the plasma LRG<\/jats:styled-content>\u2010high group, the recurrence rate tended to be higher and the recurrence\u2010free survival (RFS<\/jats:styled-content>) tended to be worse in comparison with the plasma LRG<\/jats:styled-content>\u2010low group. LRG<\/jats:styled-content> enhanced EMT<\/jats:styled-content> induced by TGF<\/jats:styled-content>\u2010\u03b2 signaling, thus indicating that LRG<\/jats:styled-content> has a significant effect on the metastasis of PDAC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/cas.13918","type":"journal-article","created":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T17:54:28Z","timestamp":1545414868000},"page":"985-996","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Epithelial\u2010mesenchymal transition via transforming growth factor beta in pancreatic cancer is potentiated by the inflammatory glycoprotein leucine\u2010rich alpha\u20102 glycoprotein<\/scp>"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7615-3124","authenticated-orcid":false,"given":"Toru","family":"Otsuru","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery Graduate 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