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We observed a change in phenotype with the suppression of MPC expression. We knocked down MPC1 and\/or MPC2 using siRNA or shRNA. We observed its cell morphology and accompanying molecular marker. Furthermore, the radioresistance of the MPC knockdown cell line was examined using a colony formation assay. MPC1\u2010suppressed cells changed their morphology to a spindle shape. Epithelial\u2010mesenchymal transition (EMT) was suspected, and examination of the EMT marker by PCR showed a decrease in E\u2010cadherin and an increase in fibronectin. Focusing on glutamine metabolism as the mechanism of this phenomenon, we knocked down the glutamine\u2010metabolizing enzyme glutaminase (GLS). EMT was also observed in GLS\u2010suppressed cells. Furthermore, when MPC1\u2010suppressed cells were cultured in a glutamine\u2010deficient medium, changes in EMT markers were suppressed. In addition, MPC1\u2010suppressed cells also increased with a significant difference in radioresistance. Decreased MPC1 expression favorably affects EMT and radioresistance of cancer.<\/jats:p>","DOI":"10.1111\/cas.13980","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T05:29:56Z","timestamp":1551158996000},"page":"1331-1339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Mitochondrial pyruvate carrier 1 expression controls cancer epithelial\u2010mesenchymal transition and radioresistance"],"prefix":"10.1111","volume":"110","author":[{"given":"Yuji","family":"Takaoka","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology Osaka University  Suita Japan"},{"name":"Department of Medical Data Science Osaka University  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6960-2315","authenticated-orcid":false,"given":"Masamitsu","family":"Konno","sequence":"additional","affiliation":[{"name":"Department of Frontier Science for Cancer and Chemotherapy Osaka University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Koseki","sequence":"additional","affiliation":[{"name":"Department of Medical Data Science Osaka University  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh","family":"Colvin","sequence":"additional","affiliation":[{"name":"Department of Medical Data Science Osaka University  Suita Japan"},{"name":"Department of Frontier Science for Cancer and Chemotherapy Osaka University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayumu","family":"Asai","sequence":"additional","affiliation":[{"name":"Department of Medical Data Science Osaka University  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Tamari","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Osaka University  Suita 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transition in cholangiocarcinoma","volume":"39","author":"Ohashi T","year":"2017","journal-title":"Oncol Rep"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38415"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.09.025"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36289"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1480085"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.18632\/oncotarget.13717","article-title":"Mitochondrial pyruvate carrier function is negatively linked to Warburg phenotype in vitro and malignant features in esophageal squamous cell carcinomas","volume":"8","author":"Li 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