{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T04:46:19Z","timestamp":1774500379171,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T00:00:00Z","timestamp":1556841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gene fusions play an important role in the carcinogenesis of lung adenocarcinoma. The recent association of four oncogenic driver genes, <jats:italic>ALK<\/jats:italic>,<jats:italic> ROS1<\/jats:italic>,<jats:italic> RET<\/jats:italic>, and <jats:italic>NTRK1<\/jats:italic>, as lung tumor predictive biomarkers has increased the need for precision medicine. We used formalin\u2010fixed, paraffin\u2010embedded tissue samples of non\u2010small cell lung cancer from 150 <jats:italic>EGFR<\/jats:italic> mutation\u2010negative cases and 10 fusion status\u2010known cases and compared the performance of the Oncomine Dx Fusion Transcript Test (ODxFT) with FISH break\u2010apart for the detection of <jats:italic>ALK<\/jats:italic>,<jats:italic> RET<\/jats:italic>, and <jats:italic>ROS1<\/jats:italic> fusion genes. RNA was extracted from the paraffin\u2010embedded tissue samples with or without macrodissection under hematoxylin and eosin staining, and the <jats:italic>ALK<\/jats:italic> fusion gene was independently determined using these assays. Fusion detection analyses were successfully carried out using ODxFT in 150 cases, with only one invalid case. <jats:italic>ALK<\/jats:italic> fusion genes were detected at a frequency of 7.3% (11\/150) in the lung cancer specimens. Concordance rate between the ODxFT and <jats:italic>ALK<\/jats:italic>\u2010FISH analyses was 99.3% (148\/149). Sensitivity and specificity were 91.7% and 99.3%, respectively. All the samples with a known fusion status were accurately matched between the two assays. Our results show a high concordance rate between the ODxFT and <jats:italic>ALK<\/jats:italic>\u2010FISH analyses. ODxFT was thus validated as an effective method for detecting clinically significant <jats:italic>ALK<\/jats:italic> fusion genes in paraffin\u2010embedded tissue samples.<\/jats:p>","DOI":"10.1111\/cas.14016","type":"journal-article","created":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T23:45:43Z","timestamp":1555026343000},"page":"2044-2049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Performance of Oncomine Fusion Transcript kit for formalin\u2010fixed, paraffin\u2010embedded lung cancer specimens"],"prefix":"10.1111","volume":"110","author":[{"given":"Kazuko","family":"Sakai","sequence":"first","affiliation":[{"name":"Department of Genome Biology Kindai University Faculty of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Ohira","sequence":"additional","affiliation":[{"name":"Department of Surgery Tokyo Medical University Tokyo 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