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Here, we demonstrated that <jats:styled-content style=\"fixed-case\">BRD<\/jats:styled-content>4 inhibitor <jats:styled-content style=\"fixed-case\">JQ<\/jats:styled-content>1 and histone deacetylase inhibitor suberoylanilide hydroxamic acid (<jats:styled-content style=\"fixed-case\">SAHA)<\/jats:styled-content> synergistically inhibited the <jats:styled-content style=\"fixed-case\">GBC<\/jats:styled-content> cells in vitro and in vivo. Our results showed that cotreatment with <jats:styled-content style=\"fixed-case\">JQ<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">SAHA<\/jats:styled-content> significantly inhibited proliferation, cell viability and metastasis, and induced apoptosis and G2\/M arrest in <jats:styled-content style=\"fixed-case\">GBC<\/jats:styled-content> cells, with only minor effects in benign cells. In vivo, tumor volumes and weights of <jats:styled-content style=\"fixed-case\">GBC<\/jats:styled-content> xenograft models were significantly decreased after treatment with <jats:styled-content style=\"fixed-case\">JQ<\/jats:styled-content>1 or <jats:styled-content style=\"fixed-case\">SAHA<\/jats:styled-content>; meanwhile, the cotreatment showed the strongest effect. Further study indicated that the above anticancer effects was associated with the downregulation of <jats:styled-content style=\"fixed-case\">BRD<\/jats:styled-content>4 and suppression of <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3K\/<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> pathways. These findings highlight <jats:styled-content style=\"fixed-case\">JQ<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">SAHA<\/jats:styled-content> as potential therapeutic agents and their combination as a promising therapeutic strategy for <jats:styled-content style=\"fixed-case\">GBC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/cas.14102","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T09:58:25Z","timestamp":1560938305000},"page":"2493-2506","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["BRD4 inhibitor and histone deacetylase inhibitor synergistically inhibit the proliferation of gallbladder cancer in vitro and in vivo"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1700-4385","authenticated-orcid":false,"given":"Shilei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of General Surgery 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